TMB Super Sensitive 1-Component HRP Microwell Substrate (SUBS) is a ready-to-use TMB substrate for microwell applications containing 3,3′,5,5′-tetramethylbenzidine in a mildly acidic buffer.
3,3′,5,5′-tetramethylbenzidine (TMB) substrates react with peroxidase, yielding a soluble blue reaction product. When the TMB-peroxidase reaction is stopped using Stop Solution for TMB Substrates (STOPT), the chromogen changes from blue to yellow, and the absorbance changes from 620 nm to 450 nm.
TMB Super Sensitive HRP Microwell Substrate is our most sensitive TMB buffer. This product is ideal for detecting very low levels of a target molecule, running samples that must be highly diluted (1:10,000), amplifying the signal when using antibodies with low binding capacity, running samples that exhibit high steric hindrance, and shortening the incubation time of the assay.
Bulk volumes are available upon request.