TMB Slow Kinetic 1-Component HRP Microwell Substrate is a ready-to-use solution containing 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. This substrate is ideal for use in all ELISAs where the target detection level is in the µg-ng/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme. Lower sensitivity substrates are suitable for ELISAs where the test samples contain high levels of the target molecule, for assays with long incubation periods (such as overnight incubations), and for assays that simply do not require a high level of sensitivity.
TMB substrate is oxidized by the peroxidase enzyme to yield a soluble blue-green reaction product. In endpoint assays, the reaction can be stopped by adding equal volumes of Stop Solution for TMB Microwell Substrates. Addition of STOPT changes the chromagen color from blue-green to yellow.
Bulk volumes are available upon request.