TMB 1-Component HRP Microwell Substrate is a ready-to-use solution containing 3,3′,5,5′-tetramethylbenzidine in a mildly acidic buffer that does not contain aprotic solvents. This substrate is ideal for use in all ELISAs where the target detection level is in the ng-pg/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme.
TMB substrate is oxidized by the peroxidase enzyme to yield a soluble blue-green reaction product. In endpoint assays, the reaction can be stopped by adding equal volumes of Stop Solution for TMB Microwell Substrates. Addition of STOPT changes the chromagen color from blue-green to yellow.
Bulk volumes are available upon request.