SynBlock ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Use of this blocking formulation minimizes backgrounds without the use of conventional cross-reactive protein additives.
Containing inert non-reactive blockers, SynBlock Blocking Buffer reduces the amount of nonspecific binding of enzyme-labeled conjugates to blocked plate surfaces. When blocking the coated immunoassay plate wells, SynBlock ELISA Blocking Buffer provides a microhydrated environment to stabilize the coated protein for better binding reactivity during the immunoassay and during long-term storage.
SynBlock works on all types of polystyrene plates except Immulon® II microplates. When blocking with SynBlock, ICT recommends Corning® 96-well EIA/RIA Stripwell™ microplates (ICT catalog #25).
SynBlock is best used to block coated ELISA plates that require high to very high blocking or that require a protein-free blocking buffer. An antimicrobial agent allows for stable room temperature blocking and long-term refrigerated storage of dried plates prepared with SynBlock. Bulk volumes and custom packaging are available upon request.