Rapid Test Mouse Monoclonal Isotyping Kit

$225.00

The ICT Rapid Test Mouse Monoclonal Isotyping Kit is a five-minute rapid lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination. This sensitive, gold-based rapid assay can be run on both tissue culture supernatant fluid and on mouse ascites fluid. This tests produces results in under 20 minutes, demonstrates increased sensitivity over latex bead assays, and tests for 6 major isotypes in one rapid test.

Catalog # Product Size Quantity Price
998 10 Tests $225.00
Catalog #: 998 Category:

The ICT Rapid Test Mouse mAb Isotyping Kit is a five-minute rapid lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination. This sensitive, gold-based rapid assay can be run on both tissue culture supernatant fluid and on mouse ascites fluid.

Determining the class and subclass of a monoclonal antibody is useful in determining the best immunoglobulin purification method. For example, IgA and IgM are often best purified by size (gel exclusion) or on immuno affinity separation columns, whereas IgG2a and IgG2b can be purified on protein A at a pH of 7 to 8. IgG1 binds best to protein A at a pH of 8 to 9. In addition, each class and isotype can be digested to Fab fragments using the appropriate amount of pepsin or other enzymes for each isotype or antibody class. Our rapid Mouse Monoclonal Antibody Isotyping Kit reduces a day-and-a-half procedure to a five-minute assay.

The Rapid Test Mouse Monoclonal Isotyping Kit is also useful for assessing the purity of a self-purified or purchased monoclonal antibody; multiple lines appear on the test strips to indicate subclass contamination.

Each Isotyping Kit contains ten pouches for testing ten samples. A total of ten cassettes for detecting IgG1, IgG2a, and IgG2b isotypes and ten cassettes for detecting IgG3, and IgM, and IgA antibodies are included. The kit also contains one 45 ml bottle of Sample Diluent.

For monoclonal antibody ascites fluid:
1. Add 0.5 µL of ascites fluid to 4 mL of Sample Diluent
2. Vortex to mix
3. Add 150 µL of diluted ascites fluid to the Sample Well.
4. Wait 5 minutes.
5. Read results. For ascites fluid, the darker red line indicates the class or subclass antibody present. Weak red lines often appear on other isotypes indicating the presence of host serum immunoglobulins in the ascites.
6. Note: Do not read after ten minutes.

For cell culture fluid:
1. Add 5.0 µL of supernatant fluid to 0.5 mL of Sample Diluent
2. Vortex to mix
3. Add 150 µL of diluted supernatant fluid to Sample Well
4. Wait 5 minutes.
5. Read results. For supernatants, a dark red line indicates which isotype or class-specific antibody is present. In very few instances, weak red lines may appear on one or more additional isotype(s) indicating multiple hybridoma clones.
6. Note: Do not read after ten minutes.

• Storage: Room temperature
• Shipping: Ships overnight (domestic), International Priority Shipping

• 10 pouches containing a total of 20 cassettes: 10 cassettes contain strips impregnated with anti-IgG1, anti-IgG2a, and anti-IgG2b isotypes; 10 cassettes contain strips that are impregnated with anti-IgG3, anti-IgM, and anti-IgA isotypes
• Isotyping Sample Diluent, 45 mL