The ICT Rapid Test Mouse mAb Isotyping Kit is a five-minute rapid lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination. This sensitive, gold-based rapid assay can be run on both tissue culture supernatant fluid and on mouse ascites fluid.
Determining the class and subclass of a monoclonal antibody is useful in determining the best immunoglobulin purification method. For example, IgA and IgM are often best purified by size (gel exclusion) or on immuno affinity separation columns, whereas IgG2a and IgG2b can be purified on protein A at a pH of 7 to 8. IgG1 binds best to protein A at a pH of 8 to 9. In addition, each class and isotype can be digested to Fab fragments using the appropriate amount of pepsin or other enzymes for each isotype or antibody class. Our rapid Mouse Monoclonal Antibody Isotyping Kit reduces a day-and-a-half procedure to a five-minute assay.
The Rapid Test Mouse Monoclonal Isotyping Kit is also useful for assessing the purity of a self-purified or purchased monoclonal antibody; multiple lines appear on the test strips to indicate subclass contamination.
Each Isotyping Kit contains ten pouches for testing ten samples. A total of ten cassettes for detecting IgG1, IgG2a, and IgG2b isotypes and ten cassettes for detecting IgG3, and IgM, and IgA antibodies are included. The kit also contains one 45 ml bottle of Sample Diluent.