pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets (SUBP)

$43.00

pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays.

 

Catalog # Product Size Quantity Price
6279 100 mL $43.00
Catalog #: 6279 Category:

pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays.

Alkaline phosphatase substrates are used in ELISA assays to detect alkaline phosphatase-conjugated molecules via a colorimetric enzymatic reaction. pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets contains para-Nitrophenylphosphate (pNPP). This formulation of pNPP substrate also contains a stabilizer to extend the shelf life of the alkaline phosphatase substrate on your benchtop to 2-3 days.

The alkaline phosphatase substrate reaction may be stopped with the use of a stop solution, Stop Solution for pNPP AP Microwell Substrates.

Bulk volumes are available upon request.

1. Run ELISA according to the specific protocol through the conjugate incubation step.
2. Wash the wells three or four times with 1X ELISA Wash Buffer (catalog #652) to remove any residual
AP-conjugate.
3. Bring SUBP to room temperature; protect from light.
4. Pipette 100 µL SUBP into each well of the plate.
5. Incubate SUBP 10-60 minutes. Monitor the color intensity.
6. Read the plate between 405-420 nm and analyze. Alternatively, stop the reaction by adding 100 µL/well STOPP (catalog #6284) and read the samples between 405-420 nm within 1 hour.

• Supplied at: 1X
• Storage: 2-8°C, protected from light