Neptune Sample Diluent

$52.00$263.00

Neptune Sample Diluent is a non-mammalian protein-containing solution highly recommended for use with serum or plasma samples from mouse, porcine or bovine sources in an antigen-down format. It can also be used for human or other mammalian plasma samples in antibody-sandwich ELISAs.

Catalog # Size Quantity Price
6124 100 mL $52.00
6125 500 mL $206.00
6126 1 L $263.00
Catalog #: 6124, 6125, 6126, 6127 Category:

Neptune Sample Diluent is a non-mammalian protein-containing solution highly recommended for use with serum or plasma samples from mouse, porcine or bovine sources in an antigen-down format. It can also be used for human or other mammalian plasma samples in antibody-sandwich ELISAs.

In certain animal species, nonspecific adsorption of serum IgG to antigen coated and blocked ELISA plate surfaces is a common problem. Utilization of Neptune Sample Diluent as the serum or plasma test sample diluent will minimize the plate well adsorption of nonspecific IgGs that are always present in serum or plasma samples.

Neptune Sample Diluent contains proprietary additives in a buffered PBS base to provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. This diluent also contains an antimicrobial agent to provide a solution free of bacterial contamination.

Bulk volumes and custom packaging are available upon request.

1. Prepare standards and samples in Neptune Sample Diluent.
2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding.
3. To dilute the sample 1:100, add 1 part sample to 99 parts Neptune Sample Diluent. For example, add 10 μL sample to 990 μL sample diluent for a total of 1,000 μL.
4. Highly concentrated samples may need to be diluted 1:1,000 or more.
5. Once diluted, run the assay according to the specific ELISA protocol.

• pH: 7.4 at 1X
• Contains: Non-mammalian Proteins
• Supplied At: 1X
• Storage: 2-8°C

Documentation

Manual
Safety Data Sheet
Certificate of Analysis