Neptune HRP Conjugate Stabilizer preserves the activity of HRP-protein conjugates. This HRP stabilizer can be used to store concentrated stock HRP conjugates, reconstitute lyophilized conjugates, and dilute HRP conjugated antibodies to their useful working titer in ELISA tests and immunology-based techniques. Neptune HRP Conjugate Stabilizer is especially suited for ELISAs using anti-IgG HRP conjugates within traditional antigen-down or antibody sandwich formats. The incorporation of hydrolyzed non-mammalian proteins in place of the traditional BSA protein base provides additional utility in situations where the presence of anti-BSA antibodies would be problematic. Additional components may be added to Neptune HRP Conjugate Stabilizer to optimize it for particular ELISA format compositions or for use in other immunological techniques. Antimicrobial agents are included to retard bacterial growth.
Neptune HRP Conjugate Stabilizer, Non-Mammalian, 1X preserves the functional integrity of the horseradish peroxidase enzyme and immunoglobulin components of stock conjugates upon reconstitution or dilution to its working concentration for ELISA or other immunology-based techniques. Its proprietary formula also stabilizes the HRP conjugate during storage and enhances the bond between detection HRP conjugated antibody and target antigen or antibody.
Non-mammalian proteins and salt additives provide structural support to both the immunoglobulin and HRP components of the conjugate, preserving enzymatic and IgG activity and stabilizing the conjugate bond, ultimately resulting in improved signal retention of HRP-conjugated antibodies.
Neptune HRP Conjugate Stabilizer maintains the specific activity of the conjugate during long-term storage. The product also inhibits the HRP conjugate from binding to nonspecific serum proteins in the sample, thereby reducing conjugate bridging on the ELISA plate surface and reducing non-specific background noise.