ICT’s HRP Goat Anti Human IgG Fc is suitable for ELISA, Western blotting, and histology techniques.
Goats were immunized with a purified preparation of human IgG Fc fragment that was obtained from a purified normal human IgG antibody protein pool. The hyperimmune IgG fraction was initially solid phase adsorbed to obtain class specificity. Subsequently, the anti-Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled human IgG Fc protein.
Affinity purified, anti-human IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-human IgG Fc prep just prior to its reaction with a 4-fold molar excess of HRP-M. This process leads to an IgG-HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule.
Using immunoelectrophoresis and ELISA analysis techniques, the IgG component of this conjugate reacts only with the Fc fragment of IgG. It does not react with kappa or lamba light chains. This conjugate is suitable for blotting, ELISA, and histology applications.
The conjugate is dissolved in a Tris based conjugate stabilization buffer containing ≤0.0085% of reaction mass of 5-chloro-2-methyl-2H-isothiazol-3-one and 2-methyl-2H-isothiazol-3-one (3:1).