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HRP Goat Anti-Human IgG Fc

$129.00

Goat Anti-Human IgG recognizes human IgG F(c) fragment. This HRP-conjugated secondary antibody was purified using antigen affinity chromatography and is suitable for ELISA, Western blotting, and histology techniques. The IgG component of this conjugate reacts only with the Fc fragment of IgG. It does not react with kappa or lamba light chains. The IgG-HRP conjugate contains between 2 and 4 HRP molecules per IgG molecule.

Catalog # Product Size Quantity Price
6291 1 mg $129.00
Catalog #: 6291 Category:

ICT’s HRP Goat Anti Human IgG Fc is suitable for ELISA, Western blotting, and histology techniques.

Goats were immunized with a purified preparation of human IgG Fc fragment that was obtained from a purified normal human IgG antibody protein pool. The hyperimmune IgG fraction was initially solid phase adsorbed to obtain class specificity. Subsequently, the anti-Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled human IgG Fc protein.

Affinity purified, anti-human IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-human IgG Fc prep just prior to its reaction with a 4-fold molar excess of HRP-M. This process leads to an IgG-HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule.

Using immunoelectrophoresis and ELISA analysis techniques, the IgG component of this conjugate reacts only with the Fc fragment of IgG. It does not react with kappa or lamba light chains. This conjugate is suitable for blotting, ELISA, and histology applications.

The conjugate is dissolved in a Tris based conjugate stabilization buffer containing ≤0.0085% of reaction mass of 5-chloro-2-methyl-2H-isothiazol-3-one and 2-methyl-2H-isothiazol-3-one (3:1).

• Molecular Weight: 230 – 310 kDa
• Recommended Dilutions:

Western blot: 1:500 – 1:2,000

ELISA: 1:5,000 – 1:20,000

IHC: 1:200 – 1:2,000

• Storage: Shelf life is 24 months from receipt when stored at 2-8°C
• Shipping: Ships overnight (domestic), International Priority Shipping