General Block contains a mammalian protein blocking agent designed to reduce nonspecific background noise in most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. This unique, low-level blocking buffer minimizes nonspecific binding interactions during the immunoassay process.
This buffer contains proprietary protein stabilizers that provide a long-term stabilizing environment for dried antigen or antibody coat proteins. Stabilization of the coated protein during short- or long-term storage results in better binding reactivity during the immunoassay, reducing the amount of necessary coat protein and increasing assay shelf life.
The mammalian General Block formulation is suitable for use in most immunoassays, including antibody sandwich ELISA, competitive assays, and peptide and protein antigen-down ELISA. An antimicrobial agent in General Block allows for stable room temperature blocking and long-term refrigerated storage dried plates.
Bulk volumes and custom packaging are available upon request.
1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8–24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT’s ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.
• pH: 7.4 at 1X
• Contains: Mammalian Proteins
• Supplied At: 1X
• Storage: 2-8°C
Boraschi-Diaz I, Tauer JT, El Rifai O, Guillemette D, Lefebvre G, Rauch F, Ferron M, Komarova SV. Metabolic phenotype in the mouse model of osteogenesis imperfecta. J. Endocrinol. 2017 Sep;234(3):279-289. doi: 10.1530/JOE-17-0335. Epub 2017 Jul 17. Abstract.
” – … 2010b). Briefly, Antibody Coating Buffer (CB1), ELISA Wash Buffer (WB1), 120 General Blocker Buffer (BB1), General Assay Diluent (AD1) and Stop Solution for TMB 121 (STOP1) were all obtained from ImmunoChemistry Technologies. 1-Step™ Ultra TMB ELISA 122 … ”