After coating the EIA/RIA plate with the capture antigen or antibody, proper blocking of the unoccupied areas of the plate wells is paramount to attaining an accurate signal. Assay sensitivity improves by utilizing the appropriate blocking buffer that effectively decreases background noise and improves the signal-to-noise ratio. Evaluation of blocking candidates is an important step of ELISA development. The Block Buffer Optimization Pack provides three of ICT’s blocking buffer formulations for an economical and fast method of selecting the best blocking buffer for a particular assay. The Pack includes 100 mL of three ELISA blocking buffers, which is adequate to begin optimizing the blocking stage of the assay development process.
Block Buffer Optimization Pack
Receive our three most popular Blocking Buffer formulations (General Block, Neptune Block, SynBlock) in this economical pack to evaluate the best blocking buffer for a particular assay.
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1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8–24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT’s ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.
• pH: 7.4 at 1X
• Contains: SynBlock – all synthetic materials; General Block – BSA; Neptune Block – non-mammalian proteins
• Supplied At: 1X
• Storage: 2-8°C