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Antigen-Down (Immunity Test) ELISA Development Kit


Assess assay feasibility and optimize ELISA performance parameters with ICT’s Antigen-Down (Immunity Test) ELISA Development Kit. Beneficial to both novice and experienced users, this kit provides seven specially formulated ELISA buffers, 96-well plates, plate storage materials, and a template for the initial development and optimization of a novel antigen-down (immunity test) format ELISA. From coating buffer to substrate and stop solutions, this kit contains enough reagent to perform ten 96-well ELISA plate assays. As an added bonus, it also includes extra plates, sealing covers, and storage materials for ten additional plates.

Catalog # Product Size Quantity Price
9101 Reagents for 10 x 96-well plates $520.00
Catalog #: 9101 Category:

An antigen-down enzyme-linked immunosorbent assay (AD ELISA) is an immunoassay-based technique that utilizes an ELISA plate coated with a target antigen to detect and/or quantify the presence of a specific antibody. Our AD ELISA Development Kit provides seven specially formulated ELISA buffers plus a detailed guide for the initial development and optimization process. AD ELISA formats are a preferred method for detecting antigen-specific antibodies that are present within serum, plasma, or cell culture supernatants (e.g., hybridoma screening). Everything from coating buffer to substrate and stop solutions are included within this AD ELISA development kit. Each kit comes with enough liquid reagents and ELISA plates to perform ten 96-well ELISA plate assays, plus ten additional plates are included for performance of some optional steps (e.g., establishing a consensus dilution factor range for samples, performing a quality assessment of the plate coating process, normalizing for signal variation over time/multiple bleed dates). Additional liquid components may be needed depending on the scale or scope of the individual AD ELISA development project.

This kit is convenient, instructive and offers an economical pathway toward achieving your goal of developing your own research project specific, novel ELISA.

Antigen-Down ELISA Development Milestones
1. Define Project Scope/Objectives and Construct Assay Development Plan.
2. Acquisition of Key Assay Components.
3. Demonstration of Basic Assay Feasibility.
4. Optimization of Assay Performance Parameters.
5. Accounting for Natural Variation in Biological Samples and Assay Conditions

• Antigen Coating Buffer, 5X, 100 mL (Catalog #6247)
• General Block, 500 mL (Catalog #633)
• General Serum Diluent, 500 mL (Catalog #648)
• Antigen-Down HRP Conjugate Stabilizer, 5X, 100 mL (Catalog #6102)
• ELISA Wash Buffer, 10X, 500 mL (Catalog #651)
• TMB 1-Component HRP Microwell Substrate, 2 x 100 mL (Catalog #6276)
• Stop Solution for TMB Substrates, 2 x 100 mL (Catalog #6282)
• Costar® 96-Well EIA/RIA Stripwell™ Plate, 20 plates (Catalog #25)
• ELISA Plate Sealing Covers, 2 x 10 pack (Catalog #6287)
• Foil ELISA Plate Storage Bags, 2 x 10 pack (Catalog #6288)
• Desiccant Packets, 2 x 10 pack (Catalog #6289)