ICT’s Assay Diluents are proprietary ELISA development additives designed to optimize antigen-down ELISA performance by equalizing any differences between the sample matrices (serum, plasma, urine, cell culture fluid) and the calibrator diluent used to generate the standard curve of the ELISA. Assay Diluents are intended to be added to each well of the plate (~100uL/well) just before the addition of the test samples.
Antigen-Down Assay Diluent is formulated for use with serum and plasma samples tested in antigen-down ELISA formats. Proprietary additives have been included in Antigen-Down Assay Diluent to reduce the level of nonspecific IgG adsorption to antigen-coated and blocked ELISA well surfaces, as well as enhancing the specific anti-analyte/antigen antibody signal without denaturing the plate adsorbed antigen molecules.
A mammalian protein-based buffer and a proprietary detergent additive are incorporated in the antigen-down formulation to minimize the nonspecific background signal that often occurs when analyzing human, porcine, or bovine serum samples. Antigen-Down Assay Diluent also contains the highest molar concentration of calcium chelating agents of all ICT assay diluent product formulations.
Formulated to inhibit interference from complement and thrombin present in serum and plasma samples, Antigen-Down Assay Diluent can also be used as a Sample Diluent for plasma sample analysis in sandwich ELISA configurations. An antimicrobial agent is included in the formulation to allow for bench-top use and storage stability.
Bulk volumes and custom packaging are available upon request.
