Alternative Block is a protein-free, detergent-free ELISA blocking buffer. This unique blocking buffer contains a heterogeneous mixture of proprietary blockers and synthetic stabilizers that block the uncoated regions of the microtiter plate without the use of conventional cross-reactive protein additives or detergents. This buffer is best for most immunoassays, including antibody-sandwich ELISA, competitive assays, and peptide and protein antigen-down ELISA.
Alternative Block’s all-synthetic heterogenous mixture of blocking agents minimizes non-specific binding interactions during the immunoassay process to reduce background noise. Alternative Block provides a microhydrated environment to stabilize the coated protein during long-term storage for improved retention of antigen epitope and antibody binding activity. When preparing batches for storage, an antimicrobial agent allows for stable room temperature blocking and long-term refrigerated storage of dried plates.