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ICT Answers Your Questions!

Question: Could you tell me the difference between MitoPT TMRE and JC-1? Answer: From a functional stand-point, JC-1, TMRM, and TMRE are all very similar. Healthy mitochondria have a charge gradient across the inner membrane; this is sometimes referred to as a...

ICT Answers Your Questions!

Question:  What is your advice for preparing stable dried ELISA plates? Answer:  In our experience we have generally found dried coated/blocked antigen plates to be more robust than dried coated/blocked antibody plates, although depending on the nature of the protein,...

ICT Answers Your Questions!

Question:  I would like to culture neuron cells and detect apoptosis using FAM FLICA kits.  I checked the adherent protocol for staining but it seemed for suspension cells.  I will perform on 24 well plate and use a microscope.  Could you direct me to a detailed and...

ICT Answers Your Questions!

Question: I would like to quantify apoptosis in living cells and I am interested in your FLICA product. Could you tell me how FLICA works? Additionally, I would like to recover the cell after incubation and use DNA for analysis. Will the FLICA assay reduce apoptosis...

ICT Answers Your Questions!

Question:  Can General Assay Diluent be used as sample/standard diluent?  Our General Assay Diluent can be used as sample/standard diluent, however the matrix is more complex than is found in a typical sample diluent, so depending on the assay you may find that it...