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In August, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of August citations from 2021.

Wang, H;Qi, W;Zou, C;Xie, Z;Zhang, M;Naito, MG;Mifflin, L;Liu, Z;Najafov, A;Pan, H;Shan, B;Li, Y;Zhu, ZJ;Yuan, J.  NEK1-mediated retromer trafficking promotes blood-brain barrier integrity by regulating glucose metabolism and RIPK1 activation. Nature communications.  2021 August 10; doi: 10.1038/s41467-021-25157-7.  Article

“Cathepsin B Activity assay MEFs were seeded in SensoPlate 24-Well Glass-Bottom Plate at the density of 50,000 cells per well and cultured for 24 h. Then cells were treated with 1X MagicRedTM (ImmunoChemistry Technologies, #937) staining solution for 30 min at 37 °C. Cells were analyzed with a Nikon Ti-E confocal microscope equipped with A1R scan head with spectral detector and resonan …”

Zhang, Y;He, L;Huang, L;Yao, S;Lin, N;Li, P;Xu, H;Wu, X;Xu, J;Lu, Y;Li, Y;Zhu, S.  Oncogenic PAX6 elicits CDK4/6 inhibitor resistance by epigenetically inactivating the LATS2‐Hippo signaling pathway.  Clinical and Translational Medicine.  2021 August 1; doi: 10.1002/ctm2.503.  Abstract

MitoPT JC-1 Assay Kit (ImmunoChemistry Technologies, Bloomington, USA) was used to examine mitochondrial membrane potential, and the procedure was conducted according to the manufacturer’s;land) according to the manufacturer’s protocol.”

Marek-Iannucci, S;Ozdemir, AB;Moreira, D;Gomez, AC;Lane, M;Porritt, RA;Lee, Y;Shimada, K;Abe, M;Stotland, A;Zemmour, D;Parker, S;Sanchez-Lopez, E;Van Eyk, J;Gottlieb, RA;Fishbein, M;Karin, M;Crother, TR;Noval Rivas, M;Arditi, M.  Autophagy-mitophagy induction attenuates cardiovascular inflammation in a murine model of Kawasaki disease vasculitis.  JCI insight.  2021 August 17; doi: 10.1172/jci.insight.151981.  Full Text

“Caspase-1 activity was measured on frozen sections of heart tissue by FAM-FLICA Caspase assay (ImmunoChemistry Technologies, #98) as previously published”

Han, M;Ishikawa, T;Stroupe, CC;Breckenridge, HA;Bentley, JK;Hershenson, MB.  Deficient inflammasome activation permits an exaggerated asthma phenotype in rhinovirus C-infected immature mice.  Mucosal immunology.  2021 August 5; doi: 10.1038/s41385-021-00436-0.  Article

“To determine caspase-1 activation, lung cells were harvested 1 day post-infection and incubated with cell-permeable FLICA 660-YVAD-FMK caspase-1 inhibitor reagent (ImmunoChemistry Technologies, Bloomington, MN).”

Gornowicz, A;Szymanowski, W;Czarnomysy, R;Bielawski, K;Bielawska, A.  Anti-HER2 monoclonal antibodies intensify the susceptibility of human gastric cancer cells to etoposide by promoting apoptosis, but not autophagy.  PloS one.  2021 August 26; doi: 10.1371/journal.pone.0255585.  Full Article

“Caspase 8 enzymatic activity assay The FAM-FLICA Caspase 8 Kit (ImmunoChemistry Technologies, USA) was used to determine caspase 8 activity after the treatment of AGS-CRL-1739 cells with etoposide (1 μM, 12.5 μM) as well as a combination of etoposide (1 μM, 12 μM) with trastuzumab or pertuzumab”

Purbasari, B;Madhyastha, R;Madhyastha, H;Nurrahmah, QI;Maruyama, M;Nakajima, Y;Kataoka, H;Watanabe, N.  Retinoic acid attenuates nuclear factor kappaB mediated induction of NLRP3 inflammasome.  Pharmacological reports : PR.  2021 August 20; doi: 10.1007/s43440-021-00321-4.  Text

“Fluorescence images were detected using a confocal laser scanning microscope TCS SP8 at 63× magnification. Pyroptosis/caspase-1 activity assay. Caspase-1 activity was assessed using Pyroptosis/caspase-1 assay kit (ImmunoChemistry Technologies, MN, USA”

Magic Red Cathepsin B Assay
MitoPT JC-1 Assay Kit
FAM FLICA Caspase-1 Assay
FAM FLICA 660 Caspase-1 Assay 
FAM FLICA Caspase-8 Assay
Pyroptosis/Caspase-1 Assay