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In July, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of July citations from 2021.

Bhagyaraj, E;Wang, H;Yang, X;Hoffman, C;Akgul, A;Goodwin, ZI;Pascual, DW.  Mucosal Vaccination Primes NK Cell-Dependent Development of CD8+ T Cells Against Pulmonary Brucella Infection.  Frontiers in immunology.  2021 July 7; doi: 10.3389/fimmu.2021.697953.  PDF

“After staining, cells were acquired on a BD Fortessa flow cytometer and analyzed by using FlowJo software. Active Caspase-1 Detection Activated caspase-1 was detected in cells by using FLICA assay (Immunochemistry Technology) as per manufacturer guidelines.”

Gorini, G;Magherini, F;Fiaschi, T;Massai, L;Becatti, M;Modesti, A;Messori, L;Gamberi, T.  Au2phen and Auoxo6, Two Dinuclear Oxo-Bridged Gold(III) Compounds, Induce Apoptotic Signaling in Human Ovarian A2780 Cancer Cells. Biomedicines.  2021 July 23; doi: 10.3390/biomedicines9080871.

“Likewise, cells were trypsinized, washed with PBS, and suspended in FAM-FLICATM caspase solution (FAM-DEVD-FMK caspase FLICA kit, ImmunoChemistry Technologies, MN 55431 USA) for 30 min at 37 °C. Cells were then washed twice with PBS and analyzed.”

Morio, A;Tsutsumi, R;Kondo, T;Miyoshi, H;Kato, T;Narasaki, S;Satomi, S;Nakaya, E;Kuroda, M;Sakaue, H;Kitamura, T;Tsutsumi, Y.  Leucine induces cardioprotection in vitro by promoting mitochondrial function via mTOR and Opa-1 signaling.  Nutrition, Metabolism and Cardiovascular Diseases.  2021 July 1; doi: 10.1016/j.numecd.2021.06.025.  Abstract

“To assess mitochondrial permeability transition pore function, MitoPT JC1 assay kit (Immunochemistry Technologies, Bloomington, MN, USA) was used. Cardiomyocytes were incubated with MitoPT JC-1 (1 g/mL) in Medium 199 for 30 min and washed ….”

Melo, AM;Mylod, E;Fitzgerald, V;Donlon, NE;Murphy, DM;Foley, EK;Bhardwaj, A;Reynolds, JV;Doherty, DG;Lysaght, J;Dunne, MR;Conroy, MJ.  Tissue distribution of γδ T cell subsets in oesophageal adenocarcinoma.  Clinical immunology (Orlando, Fla.).  2021 August 1; doi: 10.1016/j.clim.2021.108797.  Full Article

“OE33 cells were stained with CFSE (ImmunoChemistry Technologies) and seeded at a density of 200,000 cells/ml of RPMI supplemented with 10% FBS and 1% penicillin/streptomycin. Following treatments …”

Arakawa, N;Ushiki, A;Abe, M;Matsuyama, S;Saito, Y;Kashiwada, T;Horimasu, Y;Gemma, A;Tatsumi, K;Hattori, N;Tsushima, K;Saito, K;Nakamura, R;Toyoda, T;Ogawa, K;Sato, M;Takamatsu, K;Mori, K;Nishiya, T;Izumi, T;Ohno, Y;Saito, Y;Hanaoka, M.  Stratifin as a Novel Diagnostic Biomarker in Serum for Diffuse Alveolar Damage.  Research Square.  2021 July 14; doi: 10.21203/rs.3.rs-690487/v1.  PDF

“The plates were then washed three times at 25°C, and 50 µL samples (serum samples or standard SFN) diluted with General Serum Diluent (Immunochemistry Technologies, Bloomington, MN, USA) were added …”

FAM FLICA Caspase-1 Assay Kit
FAM FLICA Caspase-3/7 Assay Kit
MitoPT JC1 Assay Kit
CFSE Fluorescent Cellular Stain
General Serum Diluent