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In June, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of June citations from 2021.

Santucci, P;Greenwood, DJ;Fearns, A;Chen, K;Jiang, H;Gutierrez, MG.  Intracellular localisation of Mycobacterium tuberculosis affects efficacy of the antibiotic pyrazinamide.  Nature communications.  2021 June 21; doi: 10.1038/s41467-021-24127-3.  Article

“Stained cells were then washed twice and re-incubated in complete RPMI medium containing 1 µg/ml Hoechst 33342 (Immunochemistry Technologies, 639) and live-cell imaging was further performed using the OPERA Phenix microscope with a ×40 water-immersion objective.”

Xu, C;Sun, S;Johnson, T;Qi, R;Zhang, S;Zhang, J;Yang, K.  The glutathione peroxidase Gpx4 prevents lipid peroxidation and ferroptosis to sustain Treg cell activation and suppression of antitumor immunity.  Cell reports.  2021 June 15; doi: 10.1016/j.celrep.2021.109235.  Article

“For staining mitochondria, lymphocytes were incubated for 30 min at 37°C with 10 nM MitoTracker Deep Red (Life Technologies) or 20 nM TMRM (tetramethyl rhodamine, methyl ester; ImmunoChemistry Technologies) after staining surface markers”

Park, SS;Gonzalez-Juarbe, N;Riegler, AN;Im, H;Hale, Y;Platt, MP;Croney, C;Briles, DE;Orihuela, CJ.  Streptococcus pneumoniae binds to host GAPDH on dying lung epithelial cells worsening secondary infection following influenza.  Cell reports.  2021 June 15; doi: 10.1016/j.celrep.2021.109267.  Article

“The Cell pellets were washed three times with PBS and incubated at room temperature for 30 min with 50 μg / ml of FITC-PspA and necrotic or late apoptosis cell death was stained by propidium iodide (PI) (ImmunoChemistry Technologies, USA) according to …”

Wan, F;Zhong, G;Wu, S;Jiang, X;Liao, J;Zhang, X;Zhang, H;Mehmood, K;Tang, Z;Hu, L.  Arsenic and antimony co-induced nephrotoxicity via autophagy and pyroptosis through ROS-mediated pathway in vivo and in vitro.  Ecotoxicology and environmental safety.  2021 June 21; doi: 10.1016/j.ecoenv.2021.112442.  Full Text

“Flow cytometry (FCM) to detect pyroptotic cells. To assess pyroptosis in TCMK-1 cells, Caspase-1 activity was determined using the Pyroptosis/Caspase-1 Assay (ImmunoChemistry Technologies, USA).”

Chen, S;Luo, Z;Chen, X.  Hsa_circ_0044235 regulates the pyroptosis of rheumatoid arthritis via MiR-135b-5p-SIRT1 axis.  Cell cycle (Georgetown, Tex.).  2021 June 7; doi: 10.1080/15384101.2021.1916272.  Abstract

“Caspase-1 assay could indicate the degree of cell pyroptosis. Caspase-1 contents were assessed by flow cytometry and immunofluorescence staining. FLICA 660 Caspase-1 Assay Kit (9122, ImmunoChemistry Technologies, USA) was used to perform flow cytometry.”

Tamai, R;Mashima, I;Kiyoura, Y.  Alendronate Augments Lipid A-Induced IL-1α Release via Activation of ASC but Not Caspase-11.  Inflammation.  2021 June 2; doi: 10.1007/s10753-021-01489-w.  Abstract

“we performed a fluorochrome inhibitor of caspase assay (FLICA) using the caspase-8 staining reagent carboxyfluorescein-LETD-fluoromethylketone (FAM-LETD-FMK), according to the manufacturer’s instructions (Immunochemistry Technologies, LLC; Bloomington, MN).”

Semlali, A;Beji, S;Ajala, I;Rouabhia, M.  Effects of tetrahydrocannabinols on human oral cancer cell proliferation, apoptosis, autophagy, oxidative stress, and DNA damage.  Archives of oral biology.  2021 June 16; doi: 10.1016/j.archoralbio.2021.105200.  Text

“Following this culture period, the cells were exposed or not to 10 μM of either Δ 9 -THC or Δ 8 -THC for 24 h. In the first set of experiments, cellular ROS production was determined using a specific kit assay from ImmunoChemistry Technologies (Bloomington, MN)”

Hoechst 33342
MitoPT TMRM Assay
Propidium Iodide Stain
Pyroptosis/Caspase-1 Assay
Flica 660 Caspase-1 Assay
FAM FLICA Caspase-8 Assay
Intracellular Total ROS Activity Assay