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Our sister company, Aves Labs, has pioneered novel algorithms for finding highly-immunogenic peptide sequences within proteins, developed novel affinity purification methods specific for chicken antibodies, and developed a host of other useful reagents, such as BlokHen® blocking reagent and PrecipHen® immunoprecipitation reagent. Aves Labs continues to develop novel antibody reagents against neural-specific antigens and epitope tags to foster scientific progress in neuroscience and the molecular biology communities.

We’re happy to highlight their newest fluorescent marker, ANTI-mCHERRY ANTIBODY (mCHERRY).  The anti-mCherry chicken primary antibody is manufactured in-house. It detects mCherry, and is purified. It is provided in liquid form.  mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.  mCherry can be stored at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Volume: 100µL (MCHERRY-0020) or 500µL (MCHERRY-0100)
Concentration: 0.2mg/mL
Clonality: Polyclonal
Form: Purified
Host Species: Chicken
Antibody Isotype: IgY
Applications: ICC, WB
Gene ID: mCherry
Physical State: Liquid
Conjugate: Unconjugated
Molecular Weight: 27 kDa for native monomer
Production Notes: Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

data image from aveslabs new mcherry antibody

Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red).
Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells.
Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.

 

 

To learn more about Aves Labs and their full catalog of polyclonal chicken antibodies, click here.

NOTE:  Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.