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In February, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of February citations from 2021.

Lim, SA;Wei, J;Nguyen, TM;Shi, H;Su, W;Palacios, G;Dhungana, Y;Chapman, NM;Long, L;Saravia, J;Vogel, P;Chi, H. Lipid signalling enforces functional specialization of Treg cells in tumours.  Nature. 2021 Feb 24; doi: 10.1038/s41586-021-03235-6.  Full Article

“For mitochondrial staining, lymphocytes were incubated for 30 min at 37 °C with 10 nM MitoTracker Deep Red (Life Technologies), 20 nM tetramethylrhodamine methyl ester (TMRM; ImmunoChemistry Technologies) or 5 μM MitoSOX Red (Life Technologies) after staining of surface markers43.”

Kolb, R;De, U;Khan, S;Luo, Y;Kim, MC;Yu, H;Wu, C;Mo, J;Zhang, X;Zhang, P;Zhang, X;Borcherding, N;Koppel, D;Fu, YX;Zheng, SG;Avram, D;Zheng, G;Zhou, D;Zhang, W. Proteolysis-targeting chimera against BCL-XL destroys tumor-infiltrating regulatory T cells.  Nature Communications.  2021 Feb 24; doi:  10.1038/s41467-021-21573-x.  Article

“MC38 tumor-bearing mice were treated with 7.5 mg/kg DT2216 or vehicle via i.p. injection; 24 h later, the mice were injected with 50 µl of NIR-FLIVO 690 caspase probe (ImmunoChemistry Technologies, Bloomington, MN) via i.v. injection per the manufacturer’s suggestion. Tumors and spleens were harvested after 4 h.”

Sule, G;Abuaita, BH;Steffes, PA;Fernandes, AT;Estes, SK;Dobry, CJ;Pandian, D;Gudjonsson, JE;Kahlenberg, JM;O’Riordan, MX;Knight, JS.  Endoplasmic reticulum stress sensor IRE1α propels neutrophil hyperactivity in lupus.  The Journal of Clinical Investigation.  2021 Feb 9; doi:  10.1172/JCI137866.  Full Text

FAM-FLICA Caspase-2 Assay Kit (ImmunoChemistry Technologies) and CellEvent Caspase-3/7 Green Detection Reagent (2µM, Thermo Fisher) were used for detection of activated caspases as per manufacturers’ directions.”

Bartkowiak, K;Heidrich, I;Kwiatkowski, M;Gorges, TM;Andreas, A;Geffken, M;Verpoort, K;Müller, V;Schlüter, H;Pantel, K.  Cysteine-Rich Angiogenic Inducer 61: Pro-Survival Function and Role as a Biomarker for Disseminating Breast Cancer Cells.  Cancers.  2021 Feb 2; doi:  10.3390/cancers13030563.  Abstract

“The incubation was carried out whilst protected from light at room temperature for 15 min. The reaction was stopped by the addition of 100 µL Stop Solution for TMB Substrates (Immunochemistry Technologies, Bloomington, IN, USA) and incubation in the dark with gentle agitation for 15 min.”

Lien, T;Sun, D;Wu, C;Chang, H.  Exposure to Dengue Envelope Protein Domain III Induces Nlrp3 Inflammasome-Dependent Endothelial Dysfunction and Hemorrhage in Mice.  Frontiers in Immunology.  2021 Feb 25; doi:  10.3389/fimmu.2021.617251.  Full Text

“… ferroptosis (C11 BODIPY 581/591, Cayman Chemical, Ann Arbor, MI, USA), necroptosis (RIP3/B-2 Alexa Fluor 488, Santa Cruz Biotechnology, Santa Cruz, CA, USA), pyroptosis (Caspase-1 Assay, Green, ImmunoChemistry Technologies, MN)”

Juncker, M;Kim, C;Reed, R;Haas, A;Schwartzenburg, J;Desai, S.  ISG15 attenuates post-translational modifications of mitofusins and congression of damaged mitochondria in Ataxia Telangiectasia cells.  Biochimica et biophysica acta.  2021 Feb 19; doi:  10.1016/j.bbadis.2021.166102.  Abstract

“JC-1 staining was performed using a MitoPT™ kit (Cat# 924, Immunochemistry Technologies, Bloomington, MN). For imaging analysis, cells were grown on fibronectin-coated glass coverslips in 12-well cell culture plates. The next day, culture media was removed, and a 1× MitoPT™ JC-1 solution (2 μg/ml) was added to cover cells on the slides.”

MitoPT TMRM Assay 
NIR-FLIVO® 690 Tracer In vivo Assay 
FAM-FLICA® Caspase-2 Assay Kit 
Stop Solution for TMB Substrates 
Pyroptosis/Caspase-1 Assay, Green 
MitoPT JC-1 Assay