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In January, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of January citations from 2021.

Dufies, O;Doye, A;Courjon, J;Torre, C;Michel, G;Loubatier, C;Jacquel, A;Chaintreuil, P;Majoor, A;Guinamard, RR;Gallerand, A;Saavedra, PHV;Verhoeyen, E;Rey, A;Marchetti, S;Ruimy, R;Czerucka, D;Lamkanfi, M;Py, BF;Munro, P;Visvikis, O;Boyer, L. Escherichia coli Rho GTPase-activating toxin CNF1 mediates NLRP3 inflammasome activation via p21-activated kinases-1/2 during bacteraemia in mice. Nature Microbiology. 2021 Jan 11; doi: 10.1038/s41564-020-00832-5. Full Text

“Caspase-1 activation was detected using the fluorescent probe FAM-FLICA (ImmunoChemistry Technologies) after 6 h of treatment, according to the manufacturer’s instructions. After labelling, cells were fixed in 4% paraformaldehyde for 15 min, PFA was neutralized with 50 mM NH4Cl for 15 min, cells were permeabilized with 0.5% Triton X-100 for 5 min and blocked with 2% TBS-BSA. Cells were incubated with mouse anti-NLRP3 (clone Cryo-2, Adipogen) and/or rabbit anti-ASC (AG-25B-0006, Adipogen) or rabbit anti-phosphorylated-Pak (ab40795, Abcam) antibodies for 1 h followed…”

Lima, TS;Mallya, S;Jankeel, A;Messaoudi, I;Lodoen, MB. Toxoplasma gondii Extends the Life Span of Infected Human Neutrophils by Inducing Cytosolic PCNA and Blocking Activation of Apoptotic Caspases. mBio. 2021 Jan 26; 12(1). doi: 10.1128/mBio.02031-20. Full Article

“Active caspase-8 was quantified by using a FAM-FLICA (fluorochrome-labeled inhibitor of caspase) detection kit (FAM-LETD-FMK; ImmunoChemistry Technologies) according to the manufacturer’s instructions…”

Chen, A;Chen, Z;Zhou, Y;Wu, Y;Xia, Y;Lu, D;Fan, M;Li, S;Chen, J;Sun, A;Zou, Y;Qian, J;Ge, J. Rosuvastatin protects against coronary microembolization-induced cardiac injury via inhibiting NLRP3 inflammasome activation. Cell Death & Disease. 2021 Jan 12; 12(1)78. doi: 10.1038/s41419-021-03389-1. Full Article

“Measurement of caspase-1 activity Cells with active caspase-1 were identified using the pyroptosis/caspase-1 assay kit (Immunochemistry Technologies, Bloomington, MN, USA) according to the manufacturer’s instruction. Shortly, the H9c2 cells were trypsinized to create suspensions, and then incubated with FLICA…”

Browning, CL;Green, A;Gray, EP;Hurt, R;Kane, AB. Manganese dioxide nanosheets induce mitochondrial toxicity in fish gill epithelial cells. Nanotoxicology. 2021 Jan 27; 1-18. doi: 10.1080/17435390.2021.1874562.

“After exposure to MnCl 2 , MnO 2 nanosheets or microparticles (50 ppm Mn ion; 79.1 ug/mL MnO 2 ), cells were washed with PBS and incubated in Magic Red in vitro Cathepsin B reagent (1:32 final dilution, ImmunoChemistry Technologies) and Hoechst 33342…”

Yang, Y;He, X;Xia, S;Liu, F;Luo, L. Porphyromonas gingivalis facilitated the foam cell formation via lysosomal integral membrane protein 2 (LIMP2). Journal of Periodontal Research. 2020 Dec 29; doi: 10.1111/jre.12812.

Magic Red Cathepsin L Assay kit. Cathepsin L activity was detected with Magic Red Cathepsin L Assay Kit (ImmunoChemistry Technologies) according to the manufacturer’s recommendations. The reagent MR‐FR2 enters each cell in a non‐fluorescent state…”

LaMarche, NM. Role of Invariant Natural Killer T Cell Subsets in Adipose Tissue Homeostasis. Thesis. 2020 July 21.

“For in vivo analysis of apoptosis mice were injected I.V. with the fluorescent poly-caspase binding reagent FAM-FLIVO (Immunochemistry Technologies) and analyzed per the manufacturer’s instructions. For ex-vivo intracellular cytokine staining, suspensions of SVF cells were cultured for two hours at 37°C in complete media in the presence of Brefeldin A prior to staining with flow cytometry antibodies…”

Products Used:
FAM-FLICA Caspase-1 (YVAD) Assay
FAM-FLICA Caspase-8
Pyroptosis/Caspase-1 Assay, Green
Magic Red Cathepsin B Assay
Magic Red Cathepsin L Assay
FAM-FLIVO Poly Caspase Assay