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In October, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of October citations from 2020.

Böhme J, Martinez N, Li S, Lee A, Marzuki M, Tizazu AM, Ackart D, Frenkel JH, Todd A, Lachmandas E, Lum J, Shihui F, Ng TP, Lee B, Larbi A, Netea MG, Basaraba R, van Crevel R, Newell E, Kornfeld H, Singhal A. Metformin enhances anti-mycobacterial responses by educating CD8+ T-cell immunometabolic circuits. Nat Commun. 2020 Oct 16;11(1):5225. doi: 10.1038/s41467-020-19095-z. Full Text 

“The activity of caspase-1 was determined using fluorescent inhibitor of caspase-1 (FAM-Flica Caspase-1 Assay kit, ImmunoChemistry Technologies). Staining procedure was performed according to manufactures protocol. In brief after live/dead and surface antibody staining of lung and spleen single cells, FLICA reagent FAM-YVAD-FMK was provided to single cells and binding to activated caspase-1 was allowed for 1 h at 37 °C in the dark. Stained cells were washed with apoptosis wash buffer, followed by fixation. The green fluorescent was…”

 

Sun J, Huang Y, Gong J, Wang J, Fan Y, Cai J, Wang Y, Qiu Y, Wei Y, Xiong C, Chen J, Wang B, Ma Y, Huang L, Chen X, Zheng S, Huang W, Ke Q, Wang T, Li X, Zhang W, Xiang AP, Li W. Transplantation of hPSC-derived pericyte-like cells promotes functional recovery in ischemic stroke mice. Nat Commun. 2020 Oct 15;11(1):5196. doi: 10.1038/s41467-020-19042-y. Full Text

“Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using the in situ cell death detection kit (Roche) or fluorescently labeled inhibitor of caspases (FLICA) probe active caspase-3 (ImmunoChemistry Technologies)/NeuN coimmunostaining was applied to monitor the general level of apoptosis in the ischemic hemisphere according to the manufacturer’s instructions. Fluorescent intens;Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using the in situ cell death detection kit (Roche) or fluorescently labeled inhibitor of caspases (FLICA) probe active caspase-3 (ImmunoChemistry Technologies)/NeuN coimmunostaining was applied to monitor the general level of apoptosis in the ischemic hemisphere according to the manufacturer’s instructions. Fluorescent intens…”

 

Brempelis KJ, Cowan CM, Kreuser SA, Labadie KP, Prieskorn BM, Lieberman NAP, Ene CI, Moyes KW, Chinn H, DeGolier KR, Matsumoto LR, Daniel SK, Yokoyama JK, Davis AD, Hoglund VJ, Smythe KS, Balcaitis SD, Jensen MC, Ellenbogen RG, Campbell JS, Pierce RH, Holland EC, Pillarisetty VG, Crane CA. Genetically engineered macrophages persist in solid tumors and locally deliver therapeutic proteins to activate immune responses. J Immunother Cancer. 2020 Oct;8(2):e001356. doi: 10.1136/jitc-2020-001356. Full Text

“Tumor slices were removed from their wells and cultured in 500 µL slice culture media containing epithelial cell adhesion molecule (EpCAM)-Alexa 647 and SR-FLICA reagents (online supplemental table 1) for 3 hours on a rocker in a humidified 37°C incubation chamber. Hoechst 33342 (ImmunoChemistry Technologies) was added 15 min prior to imaging. After incubation, slices were transferred to µ-Slide 8 Well clear bottom imaging slides (Ibidi) and imaged using a Leica SP8x confocal microscope with a 10×0.4 NA dry objective. Three to four 20 uM z-stack series were obtained for each slice and used to generate maximum…”

 

Lan J, Luo H, Wu R, Wang J, Zhou B, Zhang Y, Jiang Y, Xu J. Internalization of HMGB1 (High Mobility Group Box 1) Promotes Angiogenesis in Endothelial Cells. Arterioscler Thromb Vasc Biol. 2020 Dec;40(12):2922-2940. doi: 10.1161/ATVBAHA.120.315151. Epub 2020 Oct 1. Full Text

“An hour before the indicated time points, Magic Red Cathepsin B assay reagent (Immunochemistry Technologies, MN), Magic Red Cathepsin L assay reagent (Immunochemistry Technologies), or Magic Red Cathepsin K assay reagent (Immunochemistry Technologies.”

 

Thomas M, Davis T, Nell T, Sishi B, Engelbrecht A. Amino Acid Starvation Sensitizes Resistant Breast Cancer to Doxorubicin-Induced Cell Death. Front Cell Dev Biol. 2020 Oct 15;8:565915. doi: 10.3389/fcell.2020.565915. eCollection 2020. Full Text

“The body weight of the mice was monitored twice weekly. To assess intratumour caspase cleavage, FLIVOTM in vivo apoptosis tracers (Immunochemistry Technologies LLC, MN, United States) were used. The SR FLIVOTM red dye was prepared according to the manufacturer’s protocol and 100 μl was injected into the tail vein of mice after appropriate treatments were completed. After 1 h, whole tumors were excised, digested and analyzed using flow cytometry on the BD FACSAria I.”

 

Products Used:

FAM-FLICA Caspase-1 Assay

FAM-FLICA Caspase-3/7 Assay

SR-FLICA Poly Caspase Assay

Hoechst 33342 Fluorescent Nucleic Acid Stain

Magic Red Cathepsin B Assay

SR-FLIVO in vivo Poly Caspase Assay