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In July, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of July citations from 2020.

Yin Q, Jian Y, Xu M, Huang X, Wang N, Liu Z, Li Q, Li J, Zhou H, Xu L, Wang Y, Yang C. CDK4/6 regulate lysosome biogenesis through TFEB/TFE3. J Cell Biol. 2020 Aug 3;219(8):e201911036. doi: 10.1083/jcb.201911036. Full Text

“To assess cathepsin B and cathepsin D activities, cells were treated with individual compounds for 3 h, followed by staining with Magic Red substrates (ImmunoChemistry Technologies) or BODIPY FL–pepstatin A (1 µM) according to the manufacturer’s instruction.”

 

Dodard G, Tata A, Erick TK, Jaime D, Miah SMS, Quatrini L, Escalière B, Ugolini S, Vivier E, Brossay L. Inflammation-Induced Lactate Leads to Rapid Loss of Hepatic Tissue-Resident NK Cells. Cell Rep. 2020 Jul 7;32(1):107855. doi: 10.1016/j.celrep.2020.107855. Full Text

“Apoptosis was evaluated by staining lymphocytes with Annexin V+ antibody in Annexin V binding buffer for 15 minutes at room temperature, in the dark. Additionally, apoptosis was evaluated via caspase activity using FAM-FLICA Poly Caspase Assay kit (ImmunoChemistry Technologies) according to the manufacturer’s protocol.”

 

Martín-Nalda A, Fortuny C, Rey L, Bunney TD, Alsina L, Esteve-Solé A, Bull D, Anton MC, Basagaña M, Casals F, Deyá A, García-Prat M, Gimeno R, Juan M, Martinez-Banaclocha H, Martinez-Garcia JJ, Mensa-Vilaró A, Rabionet R, Martin-Begue N, Rudilla F, Yagüe J, Estivill X, García-Patos V, Pujol RM, Soler-Palacín P, Katan M, Pelegrín P, Colobran R, Vicente A, Arostegui JI. Severe Autoinflammatory Manifestations and Antibody Deficiency Due to Novel Hypermorphic PLCG2 Mutations. J Clin. Immunol. 20 Jul 15. doi: 10.1007/s10875-020-00794-7. Online ahead of print. Full Text

“For active caspase-1 detection, PBMCs were incubated for 1 h with FLICA660 reagent (ImmunoChemistry Technologies, USA) and fixed following manufacturer recommendations.”

 

Chang Y, Zhu J, Wang D, Li H, He Y, Liu K, Wang X, Peng Y, Pan S, Huang K. NLRP3 inflammasome-mediated microglial pyroptosis is critically involved in the development of post-cardiac arrest brain injury. J Neuroinflammation. 2020 Jul 23;17(1):219. doi: 10.1186/s12974-020-01879-1. Full Text

“The bilateral cerebral cortex and hippocampus were mechanically isolated into a single-cell suspension by passage through a 40-μm cell strainer (Falcon, Madison, WI) on ice and placed in ice-cold PBS. Afterward, cells were labeled with a FAM-FLICA assay (Immunochemistry Technologies, Bloomington, MN) to detect the caspase-1 activity, in which the FLICA reagent known as FAM-YVAD-FMK forms an irreversible covalent bond with the fluoromethyl ketone (FMK) to the cleaved caspase-1. The carboxyfluorescein (FAM) optimally excites at 488–492 nm and has a peak emission at 515–535 nm.”

 

Happonen E, Tamarov K, Martikainen MV, Ketola K, Roponen M, Lehto VP, Xu W. Thermal dose as a universal tool to evaluate nanoparticle-induced photothermal therapy. Int J Pharm. 2020 Jul 16;587:119657. doi: 10.1016/j.ijpharm.2020.119657. Online ahead of print. Abstract 

“The FAM FLICA® Caspase-3/7 Kit (Immunochemistry Technologies LLC) was used to stain apoptotic cells and DRAQ7 TM (Abcam) to stain necrotic cells.”

 

Cho HJ, Yang EJ, Park JT, Kim JR, Kim EC, Jung KJ, Park SC, Lee YS. Identification of SYK inhibitor, R406 as a novel senolytic agent. Aging (Albany NY). 2020 May 7;12(9):8221-8240. doi: 10.18632/aging.103135. Epub 2020 May 7. Full Text

“Cells were then fix with 100% methanol for 10 min and washed with PBS. The Fixed cells were stained with 1 μg/ml Hoechst 33342 (#639, Immunochemistry Technologies, MN, USA) for 1 h and washed twice with PBS. Cell number was determined by measuring fluorescence intensity in Excitation/Emission: 355 nm/460 nm using the multi plate reader (Infinite M200PRO).”

 

Products Used:

Magic Red Cathepsin B Assay

FAM-FLICA Poly Caspase Assay

FLICA 660 Caspase-1 Assay

FAM-FLICA  Caspase-1 Assay

FAM-FLICA Caspase-3/7 Assay

Hoechst 33342 Fluorescent Nucleic Acid Stain