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In May, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of May citations from 2020.

Hu JJ, Liu X, Xia S, Zhang Z, Zhang Y, Zhao J, Ruan J, Luo X, Lou X, Bai Y, Wang J, Hollingsworth LR, Magupalli VG, Zhao L, Luo HR, Kim J, Lieberman J, Wu H. FDA-approved disulfiram inhibits pyroptosis by blocking gasdermin D pore formation. Nat Immunol. 2020 May 4. doi: 10.1038/s41590-020-0669-6. Online ahead of print. Abstract

“After the indicated treatments, cells were incubated with a fluorescent active caspase-1 substrate FAM-YVAD-FMK (Immunochemistry Technologies). Samples were read on a BioTek Synergy 2 plate reader.”

 

Huang Y, Wang H, Hao Y, Lin H, Dong M, Ye J, Song L, Wang Y, Li Q, Shan B, Jiang Y, Li H, Shao Z, Kroemer G, Zhang H, Bai L, Jin T, Wang C, Ma Y, Cai Y, Ding C, Liu S, Pan Y, Jiang W, Zhou R. Myeloid PTEN promotes chemotherapy-induced NLRP3-inflammasome activation and antitumour immunity. Nat Cell Biol. 2020 Jun;22(6):716-727. doi: 10.1038/s41556-020-0510-3. Epub 2020 May 4. Abstract

“Granzyme B-PE (eBioscience, 12-8898-80, NGZB, 1:100), CD44-APC (BD Pharmingen, 559250, IM7, 1:100), CD62L-PE (eBioscience, 12-0621-81, MEL-14, 1:100) were used. The FAM-FLICA-caspase-1 assay kit was obtained from Immunochemistry. Recombinant mouse IL-1β and IL-18 were obtained from MBL. The Salmonella was a gift from R.V. Bruggen. The phosphorylated-specific antibodies against mouse NLRP3 Tyr 30 (p-NLRP3) were generated by immunizing rabbits with the phosphorylated peptide CMHLEDY(p)PPEKG, covalently cross-linked to keyhole limpet haemocyanin (KLH)…”

 

Li H, Jiang W, Ye S, Zhou M, Liu C, Yang X, Hao K, Hu Q. P2Y14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. Cell Death Dis. 2020 May 26;11(5):394. doi: 10.1038/s41419-020-2609-7. Full Text

“For pyroptosis analysis, active Caspase-1 and PI fluorescence of samples were measured using flow cytometry. Active caspase-1 was detected with FLICA 660 Caspase-1 Detection Kit (ImmunoChemistry Technologies, USA) and PI staining was used to assess the integrity of cellular membrane.”

 

Song SB, Hwang ES. High Levels of ROS Impair Lysosomal Acidity and Autophagy Flux in Glucose-Deprived Fibroblasts by Activating ATM and Erk Pathways. Biomolecules. 2020 May 13;10(5):761. doi: 10.3390/biom10050761. Abstract

“To visualize lysosomes with cathepsin activity, cells were loaded with Magic Red TM (#937, ImmunoChemistry Technologies, Bloomington, CA, USA) according to the manufacturer’s protocol; after fixing, fluorescent puncta were visualized by confocal microscopy.”

 

Marschall M, Simnacher U, Walther P, Essig A, Hagemann J. The Putative Type III Secreted Chlamydia abortus Virulence-Associated Protein CAB063 Targets Lamin and Induces Apoptosis. Front Microbiol. 2020 May 25;11:1059. doi: 10.3389/fmicb.2020.01059. eCollection 2020. Full Text

“Flow cytometry served to measure cell death (propidium iodide, PI) and apoptosis (AnnexinV and fluorescent labeled inhibitors of caspase, FLICA) using the AnnexinVAPC Apoptose detection kit (BD Biosciences Europe, Darmstadt, Germany) and SR-Flica® in vitro caspase detection kit (ImmunoChemistry Technologies LCC., Bloomington, United States).”

 

Products Used:

FAM-FLICA Caspase-1 Assay

FLICA 660 Caspase-1 Assay

Magic Red Cathepsin B Assay

SR-FLICA Poly Caspase Assay