In March, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of March citations from 2020.

Cannata Serio M, Graham LA, Ashikov A, Larsen LE, Raymond K, Timal S, Le Meur G, Ryan M, Czarnowska E, Jansen JC, He M, Ficicioglu C, Pichurin P, Hasadsri L, Ríos-Ocampo WA, Gilissen C, Rodenburg R, Jonker JW, Holleboom AG, Morava E, Veltman JA, Socha P, Stevens TH, Simons M, Lefeber DJ. sMutations in the V-ATPase assembly factor VMA21 cause a congenital disorder of glycosylation with autophagic liver disease. Hepatology. 2020 Mar 7. doi: 10.1002/hep.31218. [Epub ahead of print]. Abstract

“…bottom dishes, incubated for 20 min at 37°C with Magic Red Cathepsin assay (ImmunoChemistry Technologies, LLC) or 5 min at 37°C with LysoSensor® Green DND- 189 (50 nM, Molecular Probes), diluted in DMEM medium.”

 

Reis AS, Barboza R, Murillo O, Barateiro A, Peixoto EPM, Lima FA, Gomes VM, Dombrowski JG, Leal VNC, Araujo F, Bandeira CL, Araujo RBD, Neres R, Souza RM, Costa FTM, Pontillo A, Bevilacqua E, Wrenger C, Wunderlich G, Palmisano G, Labriola L, Bortoluci KR, Penha-Gonçalves C, Gonçalves LA, Epiphanio S, Marinho CRF. Inflammasome activation and IL-1 signaling during placental malaria induce poor pregnancy outcomes. Sci Adv. 2020 Mar 4;6(10):eaax6346. doi: 10.1126/sciadv.aax6346. eCollection 2020 Mar. Full Text

“…to the active form with high affinity. BeWo and THP-1 cells exposed or nonexposed to iRBC or RBC extract, at a ratio of 20:1 (RBCs:cell) by 12-hour period, were stained for 90 min with FAM-YVAD-FMK (Immunochemistry Technologies, USA) as recommended by the manufacturer. Active caspase-1 was measured by flow cytometry. Fluorescent cell data acquisition was done using the FACSCalibur cytometer (BD B…”

 

Peng R, Zhang W, Zuo Z, Shan Y, Liu X, Tang Y, Yu Z, Wang L, Cong Y. Dimethyl sulfoxide, a potent oral radioprotective agent, confers radioprotection of hematopoietic stem and progenitor cells independent of apoptosis. Free Radic Biol Med. 2020 Mar 25;153:1-11. doi: 10.1016/j.freeradbiomed.2020.03.021. [Epub ahead of print]. Full Text 

“Apoptosis of mouse bone marrow (BM) HSCs was measured using FLICA 660 Caspase-3/7 Assay Kits (Immunochemistry Technologies, USA) following the manufacturer’s guidelines. Briefly, BMNCs prestained for HSC surface markers were resuspended in 1% BSA, added 5 uL FLICA 660 working solution and incubated at 37°C  for 30 min before flow cytometry.”

 

Neudorf H, Myette-Côté É, P Little J. The Impact of Acute Ingestion of a Ketone Monoester Drink on LPS-Stimulated NLRP3 Activation in Humans with Obesity. Nutrients. 2020 Mar 23;12(3). pii: E854. doi: 10.3390/nu12030854. Full Text

“Presence of activated caspase-1 was quantified by flow cytometry (MACSQuant Analyzer 8, Miltenyi Biotec, Bergisch Gladbach, Germany) using the FAM-FLICA® Caspase-1 Assay Kit (ImmunoChemistry Technologies, LLC, Bloomington, MN, USA) as previously described.”

 

Products Used:

Magic Red Cathepsin B Assay

FAM-FLICA Caspase-1 Assay

FLICA 660 Caspase-3/7 Assay