FOR IMMEDIATE RELEASE: February 12, 2020
ImmunoChemistry Technologies Adds Two New Products to Its Cell Viability Assays Portfolio
Bloomington, MN: ImmunoChemistry Technologies recently announced the addition of two new cell viability assays to its growing portfolio. These assays make it easier to accurately detect cell viability with easy, bright, and reliable fluorescent indicators.
The Bloomington, Minnesota-based service laboratory specializes in a wide variety of immunoassay-related services. Its assay development scientists have years of experience with protein chemistry and ELISA optimization, and this highly focused skill was used to create a Green Cathepsin B Assay, and a Far Red Pyroptosis/Caspase-1 Assay. Like other products, these two new assays are available for direct purchase from the ImmunoChemistry Technologies website.
Green Cathepsin B Assay – Cathepsins are a group of protease enzymes typically found in the lysosome. One specific cathepsin – cathepsin B – plays an important role in intracellular protein catabolism. There is evidence that increased cathepsin B activity is an indication of chronic inflammatory diseases. Elevated cathepsin activity is associated with disease states such as cancer and even Alzheimer’s disease.
The new Green Cathepsin B Assay allows researchers to detect and monitor intracellular cathepsin activity over time using live whole cells in vitro. This new assay contains the Rhodamine 110 Cathepsin B Substrate, which is a non-cytotoxic and cell membrane permeant substrate that fluoresces green upon cleavage by active cathepsins.
Pyroptosis/Caspase-1 Assay, Far Red – Pyroptosis is a highly inflammatory form of programmed cell death that occurs most frequently upon infection with intracellular pathogens and is likely to form part of the antimicrobial immune response. The National Institutes of Health report that this type of cell death is inherently inflammatory. Pyroptosis is triggered by various pathological stimuli, such as stroke, heart attack or cancer, and is crucial for controlling microbial infections.
Pyroptosis requires the function of the enzyme caspase-1. The new assay product utilizes ImmunoChemistry Technologies’ popular FLICA technology to measure caspase-1 activation. Using this kit, researchers can easily detect pyroptotic cells in their whole cell samples in vitro. FLICA probes are cell permeant noncytotoxic Fluorescent Labeled Inhibitors of CAspases that covalently bind with active caspase enzymes.
These two new additions to the portfolio of assays by ImmunoChemistry Technologies already widens the range of fluorescent whole cell assays for intracellular apoptosis detection and cellular analysis. Kits detect apoptosis, necrosis, intracellular caspase activity, cell-mediated cytotoxicity, activated serine proteases, oxidative stress, mitochondrial membrane depolarization, and more.
What sets ImmunoChemistry Technologies products apart is that the laboratory’s assay kits are designed for use in whole living cells – no lysing of the cells is required. These fluorescent assays make it easier for detection and a deeper level of accuracy.
About ImmunoChemistry Technologies: Founded in 1994, ImmunoChemistry Technologies (ICT) has grown to become a global industry leader in cell viability assays, ELISA reagents, and immunoassay services. With decades of assay and protein chemistry expertise, ICT has a wide range of products for immunoassay, cellular, and molecular diagnostics development. ICT has over 40 distributors selling products worldwide. Visit the ImmunoChemistry Technologies website to learn more about our wide range of cell viability assays, ELISA & assay reagents, and immunoassay services.