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In January, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of January citations from 2020.

Chang K, Kang P, Liu Y, Huang K, Miao T, Sagona AP, Nezis IP, Bodmer R, Ocorr K, Bai H.TGFB-INHB/activin signaling regulates age-dependent autophagy and cardiac health through inhibition of MTORC2. Autophagy. 2019 Dec 29:1-16. doi: 10.1080/15548627.2019.1704117. [Epub ahead of print]. Full Text

“Acidic organelles (including autolysosome) were monitored by staining tissue with 100 nM LysoTracker Red DND-99 (Thermo Fisher Scientific, L7528) for 5 min at room temperature. Lysosomal CtsB1 (cathepsin B1) activities were monitored using Magic Red Cathepsin-B Assay kit (ImmunoChemistry Technologies, 938) following the manufacturer’s manual. Nuclei were stained with either DAPI or Hoechst 33342 (1 µg/ml) (ImmunoChemistry Technologies, 938).”

 

Kim JK, Byun MR, Maeng CH, Kim YR, Choi JW. Selective Targeting of Cancer Stem Cells (CSCs) Based on Photodynamic Therapy (PDT) Penetration Depth Inhibits Colon Polyp Formation in Mice. Cancers (Basel). 2020 Jan 14;12(1). pii: E203. doi: 10.3390/cancers12010203. Abstract 

“To evaluate cell death in live animals, the fluorescent red FLIVO™ caspase activity probe (emission = 550-580 nm, excitation = 590-600 nm, Immunochemistry Technologies LLC, AbCys SA, Paris, France) was diluted in phosphate-buffered saline (PBS) containing…”

 

Zhou Z, Qu J, He L, Zhu Y, Yang S, Zhang F, Guo T, Peng H, Chen P, Zhou Y. Stiff matrix instigates type I collagen biogenesis by mammalian cleavage factor I complex-mediated alternative polyadenylation. JCI Insight. 2020 Feb 13;5(3). pii: 133972. doi: 10.1172/jci.insight.133972. Full Text

“To facilitate localization of the specific lung area (e.g., alveoli and fibrotic regions) and positioning of the cantilever tip, lung tissue sections were stained by a Col-F collagen-binding reagent (ImmunoChemistry Technologies) at 37°C for 1 hour followed by 2 PBS washes at room temperature.”

 

Wang Z, Sequeira RC, Zabalawi M, Madenspacher J, Boudyguina E, Ou T, Nelson JM, Nie Y, Zhao Q, Fessler MB, Zhu X. Myeloid atg5 deletion impairs n-3 PUFA-mediated atheroprotection. Atherosclerosis. 2020 Feb;295:8-17. doi: 10.1016/j.atherosclerosis.2020.01.004. Epub 2020 Jan 12. Abstract

“For active caspase-1 staining, a fluorescent peptide-based probe for active caspase-1 (FAM-YVAD-fmk; FLICA Caspase-1 Kit 98, ImmunoChemistry Technologies) was used and FLICA staining was performed as reported before [35] with minor modification.”

 

Products Used:

Magic Red Cathepsin B Assay

SR-FLIVO in vivo Poly Caspase Assay

Col-F Collagen Binding Reagent

FAM-FLICA Caspase-1 Assay