In November, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of November citations from 2019.

Miao G, Zhang Y, Chen D, Zhang H. The ER-Localized Transmembrane Protein TMEM39A/SUSR2 Regulates Autophagy by Controlling the Trafficking of the PtdIns(4)P Phosphatase SAC1. Mol Cell. 2019 Nov 25. pii: S1097-2765(19)30813-5. doi: 10.1016/j.molcel.2019.10.035. [Epub ahead of print]. Abstract 

“…MAGIC RED® CATHEPSIN B Assays ImmunoChemistry Technologies Cat# 938”

 

de Carvalho RVH, Lima-Junior DS, da Silva MVG, Dilucca M, Rodrigues TS, Horta CV, Silva ALN, da Silva PF, Frantz FG, Lorenzon LB, Souza MM, Almeida F, Cantanhêde LM, Ferreira RGM, Cruz AK, Zamboni DS. Leishmania RNA virus exacerbates Leishmaniasis by subverting innate immunity via TLR3-mediated NLRP3 inflammasome inhibition. Nat Commun. 2019 Nov 21;10(1):5273. doi: 10.1038/s41467-019-13356-2. Full Text 

“After 24 h of infection, BMDMs were stained for 1 h with FAM–YVAD–fluoromethyl ketone (FAM–YVAD–FMK; Immunochemistry Technologies), as recommended by the manufacturer’s instructions. The active Casp1 was then measured by flow cytometry. The data were acquired on a FACS ACCURI C6 flow cytometer (BD Biosciences) and analyzed with the FlowJo software (Tree Star).”

 

Tan LT, Chan CK, Chan KG, Pusparajah P, Khan TM, Ser HL, Lee LH, Goh BH. Streptomyces sp. MUM256: A Source for Apoptosis Inducing and Cell Cycle-Arresting Bioactive Compounds against Colon Cancer Cells. Cancers (Basel). 2019 Nov 6;11(11). pii: E1742. doi: 10.3390/cancers11111742. Full Text 

“Using Carboxyfluorescein FLICA Apoptosis Detection Kit (Immunochemistry technologies, LLC), the caspases-3/7 and -9 activities were measured in HCT116 cells after treatment by flow cytometer. The treated cells were harvested and incubated with 1x FAM-DEVD-FMK or FAM-LEHD-FMK reagent for 1 h at 37 °C. The stained cells were washed twice with washing solution before subjected to 10,000 events acquisition and analysis by using FACSVerseTM flow cytometer (BD Bioscience, San Jose, CA, USA).”

 

Rzepka Z, Rok J, Respondek M, Pawlik J, Beberok A, Gryko D, Wrześniok D. Cobalamin Deficiency: Effect on Homeostasis of Cultured Human Astrocytes. Cells. 2019 Nov 24;8(12). pii: E1505. doi: 10.3390/cells8121505. Full Text 

“Caspase 3/7 Assay Kit, Caspase 8 Assay Kit, and Caspase 9 Assay Kit were purchased from Sigma-Aldrich (Germany) or ImmunoChemistry Technologies (USA).”

 

Zhang M, Zhao Q, Xia M, Chen J, Chen Y, Cao X, Liu Y, Yuan Z, Wang X, Xu Y. The HDAC3 inhibitor RGFP966 ameliorated ischemic brain damage by downregulating the AIM2 inflammasome. FASEB j. 2019 Nov 26. https://doi.org/10.1096/fj.201901816R. Full Text 

“For ASC, caspase-1, AIM2 confocal staining, active caspase-1 were detected with a FLICA 660 caspase-1 assay kit according to the manufacturer’s instructions (ImmunoChemistry Technologies, Bloomington, MN, USA).”

 

Products Used:

Magic Red Cathepsin B Assay

FAM-FLICA Caspase-1 Assay

FAM-FLICA Caspase-3/7 Assay

FAM-FLICA Caspase-9 Assay

FAM-FLICA Caspase-8 Assay

FLICA 660 Caspase-1 Assay