In August, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of August citations from 2019.

Erttmann SF, Gekara NO. Hydrogen peroxide release by bacteria suppresses inflammasome-dependent innate immunity. Nat Commun. 2019 Aug 2;10(1):3493. doi: 10.1038/s41467-019-11169-x. Full Text

“FAM-YVAD-FMK FLICA (dilution 1:150) was from ImmunoChemistry Technologies.”

 

Wang X, Chang CH, Jiang J, Liu Q, Liao YP, Lu J, Li L, Liu X, Kim J, Ahmed A, Nel AE, Xia T. The Crystallinity and Aspect Ratio of Cellulose Nanomaterials Determine Their Pro-Inflammatory and Immune Adjuvant Effects In Vitro and In Vivo. Small. 2019 Aug 28:e1901642. doi: 10.1002/smll.201901642. [Epub ahead of print]. Abstract 

“After fixation and permeabilization, cells were stained with Magic Red (ImmunoChemistry Technologies) and Hoechst 33342 dye, followed by visualization under a confocal 1P/FCS inverted microscope.”

 

Umehara T, Tsujita N, Shimada M. Activation of Toll-like receptor 7/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm. PLoS Biol. 2019 Aug 13;17(8):e3000398. doi: 10.1371/journal.pbio.3000398. eCollection 2019 Aug. Full Text 

“The measurement of mitochondrial activity in sperm Sperm mitochondrial activity was measured with MitoPT JC-1 Assay Kit (911, ImmunoChemistry Technologies, llc.)”

 

Wang C, Puerta-Guardo H, Biering SB, Glasner DR, Tran EB, Patana M, Gomberg TA, Malvar C, Lo NTN, Espinosa DA, Harris E. Endocytosis of flavivirus NS1 is required for NS1-mediated endothelial hyperpermeability and is abolished by a single N-glycosylation site mutation. PLoS Pathog 2019 Jul 29;15(7):e1007938. doi: 10.1371/journal.ppat.1007938. eCollection 2019 Jul. Full Text 

“The proteolytic activity of cathepsin L was evaluated using the Magic Red assay cathepsin L detection kit (ImmunoChemistry Technologies) [9].”

 

Talty A, Deegan S, Ljujic M, Mnich K, Naicker SD, Quandt D, Zeng Q, Patterson JB, Gorman AM, Griffin MD, Samali A, Logue SE. Inhibition of IRE1α RNase activity reduces NLRP3 inflammasome assembly and processing of pro-IL1β. Cell Death Dis. 2019 Aug 16;10(9):622. doi: 10.1038/s41419-019-1847-z. Full Text

“THP-1 cells were treated as indicated and whole-cell caspase-1 activity was determined using the FLICA 660 in vitro Caspase-1 detection Kit (Immunochemistry Technologies, 9122) by the use of flow cytometry.”

 

Liverani C, De Vita A, Minardi S, Kang Y, Mercatali L, Amadori D, Bongiovanni A, La Manna F, Ibrahim T, Tasciotti E. A biomimetic 3D model of hypoxia-driven cancer progression. Sci Rep. 2019 Aug 22;9(1):12263. doi: 10.1038/s41598-019-48701-4. Full Text

“For confocal imaging, cells were washed 3 times with 1% PBS, fixed with 4% paraformaldehyde for 20 minutes at room temperature and stained with 10 µM/ml DRAQ5™ (ImmunoChemistry Technology, Bloomington, MN, USA).”

 

Products Used:

FAM-FLICA Caspase-1 Assay

Magic Red Cathepsin B Assay

MitoPT JC-1 Assay

Magic Red Cathepsin L Assay

FLICA 660 Caspase-1 Assay

DRAQ5 Far-Red Fluorescent DNA Dye