In June, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of June citations from 2019.

Göös H, Fogarty CL, Sahu B, Plagnol V, Rajamäki K, Nurmi K, Liu X, Einarsdottir E, Jouppila A, Pettersson T, Vihinen H, Krjutskov K, Saavalainen P, Järvinen A, Muurinen M, Greco D, Scala G, Curtis J, Nordström D, Flaumenhaft R, Vaarala O, Kovanen PE, Keskitalo S, Ranki A, Kere J, Lehto M, Notarangelo LD, Nejentsev S, Eklund KK, Varjosalo M, Taipale J, Seppänen MR. Gain-of-function CEBPE mutation causes non-canonical autoinflammatory inflammasomopathy. J Allergy Clin Immunol. 2019 Jun 12. pii: S0091-6749(19)30762-6. doi: 10.1016/j.jaci.2019.06.003. [Epub ahead of print]. Full Text 

“ATP (Sigma) at 5 mM was added for 20 min, followed by incubation with FAM-FLICA Caspase-1 substrate (ImmunoChemistry Technologies) for 2 h at 37 °C according to manufacturer instructions. After red blood cell lysis and staining with anti-human CD14-APC (Miltenyi Biotec) for 15 min at +4 °C, the cells were analyzed using BD Accuri C6 flow cytometer (BD Biosciences…”

 

Mulhern CM, Hong Y, Omoyinmi E, Jacques TS, D’Arco F, Hemingway C, Brogan PA, Eleftheriou D. Janus kinase 1/2 inhibition for the treatment of autoinflammation associated with heterozygous TNFAIP3 mutation. J Allergy Clin Immunol. 2019 Jun 5. pii: S0091-6749(19)30745-6. doi: 10.1016/j.jaci.2019.05.026. [Epub ahead of print]. Full Text 

“PBMCs were seeded in a 96-well plate at a density of 1.6 × 105 cells/well (8.0 × 105 cells/mL). Relevant wells were primed with 100 ng/mL LPS for 4 hours and, if required, then stimulated with 10 mmol/L ATP for 30 minutes. Caspase-1 activity was measured by using FLICA (ImmunoChemistry Technologies, Bloomington, Minn), a cell-permeable fluorescent probe (FAM-YVAD-FMK) that binds active caspase-1. Cells were incubated for 1 hour with FLICA at 37°C and stained with phycoerythrin (PE)–conjugated anti-CD14 (BD, Franklin Lakes, NJ) to identify monocytes. Cells were subsequently analyzed for the frequency of CD14+FLICA+ cells.”

 

Martínez-García JJ, Martínez-Banaclocha H, Angosto-Bazarra D, de Torre-Minguela C, Baroja-Mazo A, Alarcón-Vila C, Martínez-Alarcón L, Amores-Iniesta J, Martín-Sánchez F, Ercole GA, Martínez CM, González-Lisorge A, Fernández-Pacheco J, Martínez-Gil P, Adriouch S, Koch-Nolte F, Luján J, Acosta-Villegas F, Parrilla P, García-Palenciano C, Pelegrin P. P2X7 receptor induces mitochondrial failure in monocytes and compromises NLRP3 inflammasome activation during sepsis. Nat Commun. 2019 Jun 20;10(1):2711. doi: 10.1038/s41467-019-10626-x. Full Text  

“… in CD16−/+/++ monocytes using the monoclonal anti-P2X7 L4 clone conjugated with APC62. Active caspase-1 was measured in monocytes using the specific fluorescent probe FLICA-660 Caspase-1 Assay Kit (Immunochemistry Technologies) following the manufacturer’s instructions. Production of ROS was measured in monocytes using the red mitochondrial superoxide indicator MitoSOX (Life Technologies) following the manufacturer’s instructions.”

 

Farfel-Becker T, Roney JC, Cheng XT, Li S, Cuddy SR, Sheng ZH. Neuronal Soma-Derived Degradative Lysosomes Are Continuously Delivered to Distal Axons to Maintain Local Degradation Capacity. Cell Rep. 2019 Jul 2;28(1):51-64.e4. doi: 10.1016/j.celrep.2019.06.013. Full Text

“…1 hour to label all active GCase containing lysosomes, or with MDW933 (500 nM) applied only on the soma compartment for 15 min followed by varying washing times in NFM as indicated to label active GCase containing lysosomes delivered to distal axons. MDW933 labeled neurons were fixed and immunolabeled for b3-Tubulin as described above. For live imaging, neurons were incubated in NFM containing the various probes for 30 min at 37C, followed by 3 washes with NFM. For labeling lysosomal cargo degradation in axons, Magic Red Cathepsin B/L fluorogenic substrates (1:4,000, ImmunoChemistry Technologies) were applied to the axonal chamber for 30 min at 37C to monitor Cathepsin B/L-mediated degradation.”

 

Timilshina M, You Z, Lacher SM, Acharya S, Jiang L, Kang Y, Kim JA, Chang HW, Kim KJ, Park B, Song JH, Ko HJ, Park YY, Ma MJ, Nepal MR, Jeong TC, Chung Y, Waisman A, Chang JH. Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells. Cell Rep. 2019 Jun 4;27(10):2948-2961.e7. doi: 10.1016/j.celrep.2019.05.020. Full Text

“Mito PT TMRM ImmunoChemistry Technologies Cat#9105 Mouse recombinant IL-2 protein R and D systems Cat#202-IL-010 Mouse recombinant TGFb1 protein R and D systems Cat#240-B-010 Mouse recombinant IL-6 protein R and D systems Cat#406-ML-005 Mouse recombinant IL-12 protein R and D systems Cat#419-ML-010 Anti–mouse IL-4 Biolegend Cat#504115; RRID: AB_2295885 Anti–mouse IFN-g Biolegend Cat#517904; RRID: AB_10896754”

 

Products Used:

FAM-FLICA Caspase-1 Assay

FLICA 660 Caspase-1 Assay

Magic Red Cathepsin B Assay

Magic Red Cathepsin L Assay

MitoPT TMRM Assay