In April, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of April citations from 2019.

 

McHugh BJ, Wang R, Li HN, Beaumont PE, Kells R, Stevens H, Young L, Rossi AG, Gray RD, Dorin JR, Gwyer Findlay EL, Brough D, Davidson DJ. Cathelicidin is a “fire alarm”, generating protective NLRP3-dependent airway epithelial cell inflammatory responses during infection with Pseudomonas aeruginosa. PLoS Pathog. 2019. Apr 12;15(4):e1007694. doi: 10.1371/journal.ppat.1007694. eCollection 2019 Apr. Article

“Caspase 1 and Caspase 3/7 activity in live cells was assessed using FLICA and Magic Red fluorescent probes respectively (ImmunoChemistry Technologies, Bio-Rad AbD Serotec, Kidlington, U.K.), as per manufacturer’s instructions, in an incubator at 37˚C for 1 hour, with the addition of 1 μg/ml Hoechst (Life Technologies) for the final 30 minutes.”

 

Loomis WP, den Hartigh AB, Cookson BT, Fink SL. Diverse small molecules prevent macrophage lysis during pyroptosis. Cell Death Dis. 2019. Apr 11;10(4):326. doi: 10.1038/s41419-019-1559-4. Article

“… 2 × 10^5 cells/well (24-well plate) prior to infection with S. typhimurium or treatment with anthrax lethal toxin. Active caspase-1 was detected using a caspase-1-specific probe. FAM-YVAD-FMK (Immunochemistry Technologies) was added to a final concentration of 5 μM for the last 60 min of treatment. Cells were washed twice to remove unbound probe, then fixed and stained with the nuclear…”

 

Kawasaki K, Suzuki Y, Yamamura H, Imaizumi Y. Rapid Na+ accumulation by a sustained action potential impairs mitochondria function and induces apoptosis in HEK293 cells expressing non-inactivating Na+ channels. 2019. May 21;513(1):269-274. doi: 10.1016/j.bbrc.2019.03.129. Epub 2019 Apr 3. Abstract

“…For detection of caspase activation, SR-FLICA Poly Caspase Assay Kit (ImmunoChemistry Technologies, Bloomington, MN) was used following the protocol in the manual. Data were collected using Operetta High-Content Imaging System…”

 

Wang C, Wang R, Chen Y, Wang L, Zhou S, Wang H. Discovery of an EGFR tyrosine kinase inhibitor from Ilex latifolia in breast cancer therapy. 2019. Jun 1;29(11):1282-1290. doi: 10.1016/j.bmcl.2019.04.009. Epub 2019 Apr 4. Abstract

“… Annexin V-FITC apoptosis kit was used to measure apoptosis according to the manufacturer’s instructions (Immunochemistry Technologies). For each experiment, ≥ 20,000 cells were analyzed using a FACS flow cytometer (BD Biosciences, San Jose, CA, USA)…”

 

 

Products Used:

FAM-FLICA Caspase-1 Assay

Magic Red Caspase-3/7 Assay

SR-FLICA Poly Caspase Assay

Annexin V-FITC Apoptosis Assay