In December, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of December citations from 2018.

 

Saito T, Nah J, Oka SI, Mukai R, Monden Y, Maejima Y, Ikeda Y, Sciarretta S, Liu T, Li H, Baljinnyam E, Fraidenraich D, Fritzky, Zhai P, Ichinose S, Isobe M, Hsu CP, Kundu M, Sadoshima J. An alternative mitophagy pathway mediated by Rab9 protects the heart against ischemia. J Clin Invest. 2018 Dec 4. pii: 122035. doi: 10.1172/JCI122035. [Epub ahead of print]. Abstract

“In order to evaluate mitochondrial membrane potential/integrity, staining of cultured CMs with tetramethylrhodamine ethyl ester (TMRE) and 5,5’,6,6’- tetrachloro-1,1’,3,3’- tetraethylbenzimidazolocarbocyanine iodide (JC-1) was conducted using MitoPT® TMRE and MitoPT® JC-1 (ImmunoChemistry Technologies), respectively, according to the manufacturer’s instructions.”

 

Martínez-Fábregas J, Prescott A, van Kasteren S, Pedrioli DL, McLean I, Moles A, Reinheckel T, Poli V, Watts C. Lysosomal protease deficiency or substrate overload induces an oxidative-stress mediated STAT3-dependent pathway of lysosomal homeostasis. Nat Commun. 2018 Dec 17;9(1):5343. doi: 10.1038/s41467-018-07741-6. Full Text

“WT and AEP−/− MEF (5 × 10^4 cells) were grown on coverslips and incubated with Magic Red cathepsin L (Immunochemistry Technologies) for 60 min at 37 °C following the manufacturer’s instructions.”

 

Hafner-Bratkovič I, Sušjan P, Lainšček D, Tapia-Abellán A, Cerović K, Kadunc L, Angosto-Bazarra D, Pelegrίn P, Jerala R. NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway. Nat Commun. 2018 Dec 5;9(1):5182. doi: 10.1038/s41467-018-07573-4. Full Text

“Cells were non-treated or treated with LPS (100 ng/ml) and doxycycline (1 μg/ml) overnight. In the morning, the cells were washed and incubated with Hoechst (1 μg/ml; Immunochemistry Technologies) for 5 min at 37 °C, and washed several times before nigericin was added (5 μM).”

 

Cui Y, Carosi JM, Yang Z, Ariotti N, Kerr MC, Parton RG, Sargeant TJ, Teasdale RD. Retromer has a selective function in cargo sorting via endosome transport carriers. J Cell Biol. 2018 Dec 17. pii: jcb.201806153. doi: 10.1083/jcb.201806153. [Epub ahead of print]. Abstract

“Magic Red Cathepsin B Kit (938) was from ImmunoChemistry Technologies.”

 

Rawat P, Teodorof-Diedrich C, Spector SA. Human immunodeficiency virus Type-1 single-stranded RNA activates the NLRP3 inflammasome and impairs autophagic clearance of damaged mitochondria in human microglia. Glia 2018 Dec 24. doi: 10.1002/glia.23568. [Epub ahead of print]. Abstract

“…Culture supernatants were analyzed for LDH release by ELISA. 2.11 Active caspase‐1 measurement assay. The levels of active caspase‐1 were quantified in live cells using FLICA 660 Caspase‐1 Assay Kit (ImmunoChemistry Technologies, Bloomington, MN Cat#…”

 

Products Used:

MitoPT TMRE Assay

MitoPT JC-1 Assay

Magic Red Cathepsin L Assay

Hoechst 33342 Fluorescent Nucleic Acid Stain

Magic Red Cathepsin B Assay

FLICA 660 Caspase-1 Assay