In September, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of September citations from 2018.

 

Inzaugarat ME, Johnson CD, Holtmann TM, McGeough MD, Trautwein C, Papouchado BG, Schwabe R, Hoffman HM, Wree A, Feldstein AE. NLRP3 inflammasome activation in hepatic stellate cells induces murine liver fibrosis. Hepatology. 2018. Sep 4. doi: 10.1002/hep.30252. [Epub ahead of print]. Abstract

“For detecting cathepsin B activity, stimulated hepatic stellate cells were stained using cell – permeable fluorescently labeled Magic Red cathepsin B substrate (ImmunoChemistry Technologies, Bloomington, MN) and Hoechst stain according to the manufacturer’s…”

 

Qiu T, Pei P, Yao X, Jiang L, Wei S, Wang Z, Bai J, Yang G, Gao N, Yang L, Qi S, Yan R, Liu X, Sun X. Taurine attenuates arsenic-induced pyroptosis and nonalcoholic steatohepatitis by inhibiting the autophagic-inflammasomal pathway. Cell Death Dis. 2018. Sep 20;9(10):946. doi: 10.1038/s41419-018-1004-0. Full Text

“…Scores of 0–2 are not NASH, 3–4 are considered borderline, and 5–8 are considered NASH8. Pyroptosis detection Liver frozen slide The pyroptotic cells were detected with FLICA® 660-YVAD-FMK (ImmunoChemistry Technologies, NRM-KF17362) by immunofluorescence, according to the manufacturer’s protocol. The surfaces of the tissues were covered in tissue section staining solution (TSSS) and in…”

 

Ebrahimi T, Rust M, Kaiser SN, Slowik A, Beyer C, Koczulla AR, Schulz JB, Habib P, Bach JP. α1-antitrypsin mitigates NLRP3-inflammasome activation in amyloid β1-42-stimulated murine astrocytes. J Neuroinflammation. 2018. Sep 27;15(1):282. doi: 10.1186/s12974-018-1319-x. Full Text

“…FAM-FLICA® Caspase-1 Assay Kit (ImmunoChemistry Technologies, USA) was performed according to the manufacturer’s protocol to detect caspase 1 activity after 3 h and 6 h stimulation time…”

 

Suschak JJ, Bagley K, Six C, Shoemaker CJ, Kwilas S, Spik KW, Dupuy LC, Schmaljohn CS. The genetic adjuvant IL-12 enhances the protective efficacy of a DNA vaccine for Venezuelan equine encephalitis virus delivered by intramuscular injection in mice. Antiviral Res. 2018. Nov;159:113-121. doi: 10.1016/j.antiviral.2018.09.014. Epub 2018 Sep 27. Abstract

“… at −80 °C. ELISA plates were coated at a concentration of 150 ng/well, and then incubated overnight at 4 °C. The following day, plates were washed with PBS containing 0.2% Tween-20 and then blocked with Neptune Block (ImmunoChemistry Technologies, Bloomington…”

 

Rusin A, Lapeid E, Le M, Seymour C, Oughton D, Haanes H, Mothersill C. Effect of gamma radiation on the production of bystander signals from three earthworm species irradiated in vivo. Environmental Research. 2018. Sep 20;168:211-221. doi: 10.1016/j.envres.2018.09.023. [Epub ahead of print]. Full Text

“…Specifically, the The MitoPT® JC-1 Assay (Immunochemistry Technologies, Bloomington, USA) was used A full explanation of the dye and preparation of the dye solution used can be found in the JC1 assay manual (Immunochemistry Technologies, Bloomington, USA…”

 

Products Used:

Magic Red Cathepsin B Assay

FLICA 660 Caspase-1 Assay

FAM-FLICA Caspase-1 Assay

Neptune Block ELISA Blocking Buffer

MitoPT JC-1 Assay