In August, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of August citations from 2018.

 

Zhang J, Wang J, Zhou Z, Park JE, Wang L, Wu S, Sun X, Lu L, Wang T, Lin Q, Sze SK, Huang D, Shen HM. Importance of TFEB acetylation in control of its transcriptional activity and lysosomal function in response to histone deacetylase inhibitors. Autophagy. 2018;14(6):1043-1059. doi: 10.1080/15548627.2018.1447290. Epub 2018 Jul 30. Abstract

“…Magic Red® Cathepsin B/CTSB, Magic Red® Cathepsin L/ CTSL and Acridine Orange Staining Solution (Immunochemistry Technologies, LLC, 937, 942 and 6130, respectively), DQTM Red BSA (Molecular Probes, D12051), Click-iT® AHA (Lazidohomoalanine) reagent (Invitrogen, C10289)…”

 

Smith JNP, Zhang Y, Li JJ, McCabe A, Jo HJ, Maloney J, MacNamara KC. Type I IFNs drive hematopoietic stem and progenitor cell collapse via impaired proliferation and increased RIPK1-dependent cell death during shock-like ehrlichial infection. PLoS Pathog. 2018 Aug 6;14(8):e1007234. doi: 10.1371/journal.ppat.1007234. eCollection 2018 Aug. Full Text

“Caspase activity was assayed by incubating freshly isolated bone marrow cells with FAM-LETD-FMK caspase 8 and FAM-DEVD-FMK caspase 3/7 fluorescent inhibitor probes (ImmunoChemistry Technologies) according to manufacturer’s instructions. Caspase activity was analyzed within viable cell populations.”

 

Ahlstrand T, Torittu A, Elovaara H, Välimaa H, Pöllänen MT, Kasvandik S, Högbom M, Ihalin R. Interactions between the Aggregatibacter actinomycetemcomitans secretin HofQ and host cytokines indicate a link between natural competence and interleukin-8 uptake. Virulence. 2018;9(1):1205-1223. doi: 10.1080/21505594.2018.1499378. Full Text

“Empty binding sites were blocked with 200 μl of Alternative Block (BB5, ImmunoChemistry Technologies, #6299) at RT overnight, after which the wells were washed 3 times as described above. Serial dilutions of C-His-emHofQ (from 10 to 740 nM) in Delfia Assay Buffer were incubated in wells at RT for 1 h, after which the plate was washed as described above…”

 

Grasso E, Gori S, Soczewski E, Fernández L, Gallino L, Vota D, Martínez G, Irigoyen M, Ruhlmann C, Lobo TF, Salamone G, Mattar R, Daher S, Leirós CP, Ramhorst R. Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells. Sci Rep. 2018 Aug 16;8(1):12274. doi: 10.1038/s41598-018-29779-8. Full Text

“For determination of caspase-1 activation, FLICA reagent was added in Non-dec and Dec cells cultures, according to manufacturer’s instructions (Immunochemistry, Technologies, USA). Briefly, at day 8 of decidualization, cells were washed and incubated with FLICA-probe for 2 h in DMEM-F12 10% FBS.”

 

Cavassani KA, Meza RJ, Habiel DM, Chen JF, Montes A, Tripathi M, Martins GA, Crother TR, You S, Hogaboam CM, Bhowmick N, Posadas EM. Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells. Cancer Med. 2018 Aug 9. doi: 10.1002/cam4.1695. [Epub ahead of print]. Full Text

“Caspase-1 activation was detected by flow cytometry using FLICA assay (ImmunoChemistry Technologies, Bloomington, MN, USA) according to manufacturer’s instructions.”

 

Products Used:

Magic Red Cathepsin B Assay

Magic Red Cathepsin L Assay

Acridine Orange

FAM-FLICA Caspase-8 Assay

FAM-FLICA Caspase-3/7 Assay

Alternative Block

FAM-FLICA Caspase-1 Assay