In July, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of July citations from 2018.

 

Akkaya M, Traba J, Roesler AS, Miozzo P, Akkaya B, Theall BP, Sohn H, Pena M, Smelkinson M, Kabat J, Dahlstrom E, Dorward DW, Skinner J, Sack MN, Pierce SK. Second signals rescue B cells from activation-induced mitochondrial dysfunction and death. Nat Immunol. 2018 Jul 9. doi: 10.1038/s41590-018-0156-5. [Epub ahead of print]. Abstract

“Analysis of different stages of cell death was carried out using a necrosis vs apoptosis kit (Immunochemistry Technologies) according to the manufacturer’s protocol.”

 

Manna S, Howitz W, Oldenhuis N, Eldredge A, Shen J, Nihesh F, Lodoen M, Guan Z, Esser-Kahn A. Immunomodulation of the NLRP3 Inflammasome through Structure-Based Activator Design and Functional Regulation via Lysosomal Rupture. ACS Cent Sci. 2018 Jul 2. doi: 10.1021/acscentsci.8b00218. Full text

“Lysoview 633 was obtained from Biotium. APG-4 was purchased from TEFLabs, monensin from Tocris, and filipin-III from SigmaAldrich. Propidium iodide, FAM-YVAD-FMK kit (FAM FLICA Caspase-1), and cathepsin B MR-(RR)2 kit (Magic Red cathepsin B) were bought from Immunochemistry Technologies.”

 

Rissiek B, Lukowiak M, Raczkowski F, Magnus T, Mittrücker HW, Koch-Nolte F. In Vivo Blockade of Murine ARTC2.2 During Cell Preparation Preserves the Vitality and Function of Liver Tissue-Resident Memory T Cells. Front Immunol. 2018 Jul 9;9:1580. doi: 10.3389/fimmu.2018.01580. eCollection 2018. Full text

“Cells were incubated for 2 h at 4°C on ice or at 37°C in a cell culture incubator in the presence of propidium iodide (PI, 2.5 µg/ml, ImmunoChemistry Technologies, LLC). PI uptake was used to determine cell death by flow cytometry directly after incubation.”

 

Suschak JJ, Bagley K, Shoemaker CJ, Six C, Kwilas S, Dupuy LC, Schmaljohn CS. The Genetic Adjuvants Interleukin-12 and Granulocyte-Macrophage Colony Stimulating Factor Enhance the Immunogenicity of an Ebola Virus Deoxyribonucleic Acid Vaccine in Mice. J Infect Dis. 2018 Jul 19. doi: 10.1093/infdis/jiy378. [Epub ahead of print]. Full text 

“The ELISA plates were incubated overnight at 4°C. The following day, plates were washed with PBS containing 0.2% Tween 20 and then blocked with Neptune Block (ImmunoChemistry Technologies, Bloomington, MN) for 2 hours at 37°C.”

 

Schuster S, Johnson C, Hennebelle M, Holtmann T, Taha A, Kirpich I, Eguchi A, Ramsden C, Papouchado B, McClain C, Feldstein A. Oxidized linoleic acid metabolites induce liver mitochondrial dysfunction, apoptosis and NLRP3 activation in mice. J Lipid Res. 2018 Jul 3. pii: jlr.M083741. doi: 10.1194/jlr.M083741. [Epub ahead of print]. Full text

“To detect Caspase-1 activity in frozen liver tissue slides, we used the FAM FLICA Caspase-1 Assay Kit (ImmunoChemistry Technologies, Bloomington, MN, USA) according to manufacturer’s instructions.”

 

Ha SD, Solomon O, Akbari M, Sener A, Kim SO. Histone deacetylase 8 protects human proximal tubular epithelial cells from hypoxia-mimetic cobalt- and hypoxia/reoxygenation-induced mitochondrial fission and cytotoxicity. Sci Rep. 2018 Jul 27;8(1):11332. doi: 10.1038/s41598-018-29463-x. Full text 

“TMRM was used to measure ΔΨm. Cells exposed to cobalt or H/R were washed with pre-warmed fresh media and stained with TMRM (200 nM) for 30 min at 37 °C according to the ImmunoChemistry Technologies, MitoPT® TMRM assay protocol.”

 

 

Products Used:

Necrosis vs Apoptosis Assay

FAM-FLICA Caspase-1 Assay

Magic Red Cathepsin B Assay

Propidium Iodide Stain

Neptune Block ELISA Blocking Buffer

MitoPT TMRM Assay