In June, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of June citations from 2018.

 

Lau A, Chung H, Komada T, Platnich JM, Sandall CF, Choudhury SR, Chun J, Naumenko V, Surewaard BG, Nelson MC, Ulke-Lemée A, Beck PL, Benediktsson H, Jevnikar AM, Snelgrove SL, Hickey MJ, Senger DL, James MT, Macdonald JA, Kubes P, Jenne CN, Muruve DA. Renal immune surveillance and dipeptidase-1 contribute to contrast-induced acute kidney injury. J Clin Invest. 2018 Jun 4. pii: 96640. doi: 10.1172/JCI96640. [Epub ahead of print]. Full text

“THP-1 cells were differentiated with PMA on coverslips and treated with various reagents. Cells were incubated with FAM-YVAD-fmk (FLICA–caspase-1) (ImmunoChemistry Technologies) for 1 hour before fixation with 4% paraformaldehyde. Incubation with NH4Cl (50 μM) was used to quench autofluorescence before permeabilization with 0.1% Triton X-100.”

 

He B, Shi Y, Liang Y, Yang A, Fan Z, Yuan L, Zou X, Chang X, Zhang H, Wang X, Dai W, Wang Y, Zhang Q. Single-walled carbon-nanohorns improve biocompatibility over nanotubes by triggering less protein-initiated pyroptosis and apoptosis in macrophages. Nat Commun 2018 Jun 19;9(1):2393. doi: 10.1038/s41467-018-04700-z. Full text

“Necrostatin-1 (NEC-1) was provided by Cayman Chemical (Ann Arbor, MI, USA). Bafilomycin A (Baf A) and trypsin (0.25%) were purchased from Keygen Biotech (Jiangsu, China). N-Acetyl-L-Cysteine (NAC), Cytochalasin D (Cyto D), cholesterol, DiI, Coumarin-6, Nile red, Pyrene, 1,6-Diphenyl-1,3,5-hexatriene (DPH) were obtained from Aladdin (Shanghai, China). Magic Red Cathepsin B Detection kit (Catalog No 937) was obtained from ImmunoChemistry Technologies (Bloomington, MN, USA).”

 

Yap CC, Digilio L, McMahon LP, Garcia ADR, Winckler B. Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes. J Cell Biol. 2018 Jun 15. pii: jcb.201711039. doi: 10.1083/jcb.201711039. [Epub ahead of print]. Abstract

“We used MR-Cat assay kits (1:1,300 dilution; 937, 939, and 941; Immunochemistry Technologies) to live label active CatB, K, and L in transfected neurons according to the manufacturer’s protocol.”

 

Xu W, Tamarov K, Fan L, Granroth S, Rantanen J, Nissinen T, Peräniemi S, Uski O, Hirvonen MR, Lehto VP. A Scalable Synthesis of Biodegradable Black Mesoporous Silicon Nanoparticles for Highly Efficient Photothermal Therapy. ACS Appl Mater Interfaces. 2018 Jul 2. doi: 10.1021/acsami.8b04557. [Epub ahead of print]. Abstract

“Cell viability was examined with CellTiter Glo® assay and the apoptotic and necrotic cells were analyzed with flow cytometry using FAM FLICA® Caspase-3/7 Kit (Immunochemistry Technologies LLC) to stain apoptotic cells and DRAQ7TM (Abcam) to stain necrotic cells.”

 

Adutler-Lieber S, Friedman N, Geiger B. Expansion and Antitumor Cytotoxicity of T-Cells Are Augmented by Substrate-Bound CCL21 and Intercellular Adhesion Molecule 1. Front Immunol. 2018 Jun 11;9:1303. doi: 10.3389/fimmu.2018.01303. Full text

“A microscopic cell-counting assay was performed using automated analysis of fluorescent microscope images. Live cells were identified by staining the cell nuclei with 1 µg/ml Hoechst (33342, ImmunoChemistry Technologies, Bloomington, MN, USA) and with 250 ng/ml propidium iodide (Sigma Aldrich, St. Louis, MO, USA), for elimination of dead cells from the analysis.”

 

Products Used:

FAM-FLICA Caspase-1 Assay

Magic Red Cathepsin B Assay

Magic Red Cathepsin K Assay

Magic Red Cathepsin L Assay Kit

FAM-FLICA Caspase-3/7 Assay

Hoechst 33342 Fluorescent Nucleic Acid Stain