In May, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of May citations from 2018.

 

Wang X, Lee JH, Li R, Liao YP, Kang J, Chang CH, Guiney LM, Mirshafiee V, Li L, Lu J, Xia T, Hersam MC, Nel AE. Toxicological Profiling of Highly Purified Single-Walled Carbon Nanotubes with Different Lengths in the Rodent Lung and Escherichia Coli. Small. 2018 Jun;14(23):e1703915. doi: 10.1002/smll.201703915. Epub 2018 May 7.

“Confocal Microscopy to Assess Lysosomal Damage and Cathepsin B Release: A total of 1 × 10^5 primed THP-1 cells were seeded into each well of an eight-well chamber and incubated with 50 µg mL−1 SWCNTs for 24 h. After fixation in 4% paraformaldehyde for 1 h in phosphate buffer solution (PBS), cells were washed three times with PBS and stained with Magic Red Cathepsin B (ImmunoChemistry Technologies) at 26 × 10−9 M for 1 h.” Abstract

 

Zeng Q, Fu J, Korrer M, Gorbounov M, Murray PJ, Pardoll D, Masica DL, Kim YJ. Caspase-1 from Human Myeloid-Derived Suppressor Cells Can Promote T Cell-Independent Tumor Proliferation. Cancer Immunol Res. 2018 May;6(5):566-577. doi: 10.1158/2326-6066.CIR-17-0543. Epub 2018 Apr 13. Full text

“Fluorescent-labeled inhibitors of caspase-1 (FLICA) covalently bind to active caspase enzymes in cells. Caspase-1–specific FLICA 660 Assay kit (ImmunoChemistry Technologies) was used to stain the sorted human MDSCs at 37°C for 1 hour, then washed 2 times with buffer provided in the kit, and counterstained with DAPI, per the assay protocol.”

 

Zhang J, Wang J, Wong YK, Sun X, Chen Y, Wang L, Yang L, Lu L, Shen H, Huang D. Docetaxel enhances lysosomal function through TFEB activation. Cell Death Dis. 2018 May 23;9(6):614. doi: 10.1038/s41419-018-0571-4. Full text

“The chemicals used in our experiments were: Annexin V Pacific Blue™ conjugate (Thermo Fisher Scientific, A35122), acridine orange (AO) (Immunochemistry Technologies, LLC, 6130), docetaxel (Sigma, 01885), chloroquine (CQ) (PubChem, 2719), lysoTracker Red DND-99 (Invitrogen, L7528), lysoSensor green DND-189 (Invitrogen, L7535), Magic Red™ cathepsin B and L reagent with Acridine Orange (Immunochemistry Technologies, LLC, 937/938/6130), thapsigargin (Sigma, T9033).”

 

Chen L, Zhao Y, Lai D, Zhang P, Yang Y, Li Y, Fei K, Jiang G, Fan J. Neutrophil extracellular traps promote macrophage pyroptosis in sepsis. Cell Death Dis. 2018 May 22;9(6):597. doi: 10.1038/s41419-018-0538-5. Full text

“PMϕ/BMDM (5 × 10^5 cells) cultured in 35 mm Petri dishes were stimulated with NETs (200 μl) for 9 h, followed by co-culturing with DQ Ovalbumin (10 mg/ml; Molecular Probes) or Magic Red CatB assay reagent (10 mg/ml; Immunochemistry Technologies, Bloomington, MN, USA) at 37 °C for 1 h for the detection of lysosome rupture and CatB activation.”

 

Michels S, Ganjam GK, Martins H, Schratt GM, Wöhr M, Schwarting RKW, Culmsee C. Downregulation of the psychiatric susceptibility gene Cacna1c promotes mitochondrial resilience to oxidative stress in neuronal cells. Cell Death Discov. 2018 May 10;4:54. doi: 10.1038/s41420-018-0061-6. eCollection 2018. Full text

“Mitochondrial membrane depolarization was determined using the MitoPT TMRE Kit (ImmunoChemistry Technologies, Hamburg, Germany) following the recommendations of the manufacturer. HT22 cells were collected and stained with 0.2 µM TMRE (tetramethylrhodamine ethyl ester) for 30 min at 37 °C. The depolarizing reagent CCCP (carbonyl cyanide 3-chlorophenylhydrazone) was used in a final concentration of 50 µM and served as additional positive control.”

 

Products Used:

Magic Red Cathepsin B Assay

FAM-FLICA Caspase-1 Assay

Magic Red Cathepsin L Assay Kit

Acridine Orange Staining Solution

MitoPT TMRE Assay