In March, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of March citations from 2018.

 

Ou J, Ball JM, Luan Y, Zhao T, Miyagishima KJ, Xu Y, Zhou H, Chen J, Merriman DK, Xie Z, Mallon BS, Li W. iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications. Cell. 2018 Mar 20. pii: S0092-8674(18)30290-3. doi: 10.1016/j.cell.2018.03.010. [Epub ahead of print]. Abstract

“…GS and Human iPSC-neuronal cultures were prepared in two groups and incubated with hibernate-medium containing Magic Red (1:1000; ImmunoChemistry Technologies, Bloomington, MN) at 37 C for 25 min, followed by the addition of LysoTracker Green DND-26 (50 nM; Thermo Fisher, Waltham, MA; see Key Resources Table) for 5 min…”

 

Mirshafiee V, Sun B, Chang CH, Liao YP, Jiang W, Jiang J, Liu X, Wang X, Xia T, Nel AE. Toxicological Profiling of Metal Oxide Nanoparticles in Liver Context Reveals Pyroptosis in Kupffer Cells and Macrophages versus Apoptosis in Hepatocytes. ACS Nano. 2018 Mar 19. doi: 10.1021/acsnano.8b01086. [Epub ahead of print]. Abstract

“…The FAM-FLICA Caspase-1, Caspase-3/7, and Magic Red Cathepsin B Assay Kits were purchased from ImmunoChemistry Technologies, LLC (Bloomington, MN)…”

Hansen IS, Krabbendam L, Bernink JH, Loayza-Puch F, Hoepel W, van Burgsteden JA, Kuijper EC, Buskens CJ, Bemelman WA, Zaat SAJ, Agami R, Vidarsson G, van den Brink GR, de Jong EC, Wildenberg ME, Baeten DLP, Everts B, den Dunnen J. FcαRI co-stimulation converts human intestinal CD103+dendritic cells into pro-inflammatory cells through glycolytic reprogramming. Nat Commun. 2018 Feb 28;9(1):863. doi: 10.1038/s41467-018-03318-5. Full text

“…Caspase-1 activation was determined using caspase-1 binding compound FAM-YVAD-FMK from FAM-FLICA Caspase-1 Assay Kit (ICT098; ImmunoChemistry Technologies) according to manufacturer’s instructions. Cells were assessed for fluorescence using flow cytometry (Canto II, BD Biosciences)…”

 

Luan H, Mohapatra B, Bielecki TA, Mushtaq I, Mirza S, Jennings TA, Clubb RJ, An W, Ahmed D, El Ansari R, Storck MD, Mishra NK, Guda C, Sheinin YM, Meza JL, Raja S, Rakha EA, Band V, Band H. Loss of the nuclear pool of ubiquitin ligase CHIP/STUB1 in breast cancer unleashes the MZF1-cathepsin pro-oncogenic program. Cancer Res. 2018 Mar 6. pii: canres.2140.2016. doi: 10.1158/0008-5472.CAN-16-2140. [Epub ahead of print]. Full text

“…CTSB/L activity was assayed using the Magic Red CTSB/L Activity Kit (Cat. 937 & 941, Immunochemistry Technologies, Bloomington, MN) according to the manufacturer’s protocol (See Supplementary Methods). Experiments were run in triplicates and repeated thrice…”

 

Watson DC, Moysi E, Valentin A, Bergamaschi C, Devasundaram S, Fortis SP, Bear J, Chertova E, Bess J Jr., Sowder R, Venzon DJ, Deleage C, Estes JD, Lifson JD, Petrovas C, Felber BK, PavlakisGN. Treatment with native heterodimeric IL-15 increases cytotoxic lymphocytes and reduces SHIV RNA in lymph nodes. PLoS Pathog. 2018 Feb 23;14(2):e1006902. doi: 10.1371/journal.ppat.1006902. eCollection 2018 Feb. Full text

“…the fluorescent 660-DEVD-FMK reagent, an inhibitor of caspase 3 and caspase 7 that diffuses into the cells and irreversibly binds to the active forms of the caspases (FLICA assay, ImmunoChemistry Technologies) was added to the samples. After 1 hour, the samples were washed with the FLICA kit buffer and analyzed for apoptosis by flow cytometry. Multiparameter confocal imaging…”

 

Lamb LS, Pillai S, Langford S, Bowersock J, Stasi AD, Saad A. Clinical-scale manufacturing of γδ T cells for protection against infection and disease recurrence following haploidentical peripheral blood stem cell transplantation and cyclophosphamide gvhd prophylaxis. Bone Marrow Transplant. 2018 Mar 7. doi: 10.1038/s41409-018-0130-8. [Epub ahead of print] No abstract available. Correspondence

“…Potency was measured by cytotoxic activity against K562 erythroleukemia target cells using the ImmunoChemistry Technologies (ICT) Basic Cytotoxicity Kit…”

 

Ueshima C, Kataoka TR, Hirata M, Sugimoto A, Iemura Y, Minamiguchi S, Nomura T, Haga H. Possible involvement of human mast cells in the establishment of pregnancy via KIR2DL4. Am J Pathol. 2018 Mar 22. pii: S0002-9440(17)30912-4. doi: 10.1016/j.ajpath.2018.02.012. [Epub ahead of print]. Abstract

“…The anti-KIR2DL4 antibody-stimulated LAD2 cells or the co-cultured LAD2 and HTR-8/SVneo cells were collected, and their serine protease activity was measured using a FAM-Leu-CMK Green FLISP Assay Kit (Immunochemistry Technologies, Bloomington, MN)…”

 

Products Used:

Magic Red Cathepsin B Assay

Magic Red Cathepsin L Assay Kit

FAM-FLICA Caspase-1 Assay

FAM-FLICA Caspase-3/7 Assay

FLICA 660 Caspase-3/7 Assay Kit

Cell-mediated Cytotoxicity Assay: Basic Cytotoxicity Assay

FLISP FAM-Leu-CMK Serine Protease Assay Kit