In February, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of February citations from 2018.

Chennupati V, Veiga DF, Maslowski KM, Andina N, Tardivel A, Yu EC, Stilinovic M, Simillion C, Duchosal MA, Quadroni M, Roberts I, Sankaran VG, MacDonald HR, Fasel N, Angelillo-Scherrer A, Schneider P, Hoang T, Allam R. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 mRNA translation. J. Clin. Invest. 2018 Mar 19. pii: 94956. doi: 10.1172/JCI94956. [Epub ahead of print]. Full text

” … (PE), APC from eBiosciences. CD34 (AC136) antibody from Miltenyi Biotec. Hoechst 33342 fluorescent nuclear stain (ImmunoChemistry Technologies) was added at a dilution of 1:200 at least 15 minutes before analysis. Data were acquired on …”

 

Templeman NM, Luo S, Kaletsky R, Shi C, Ashraf J, Keyes W, Murphy CT. Insulin Signaling Regulates Oocyte Quality Maintenance with Age via Cathepsin B Activity. Curr. Biol. 2018 Mar 5;28(5):753-760.e4. doi: 10.1016/j.cub.2018.01.052. Epub 2018 Feb 22. Abstract

” … RNeasy Mini Kit, QIAGEN, Cat# 74104. Agilent Quick-Amp Labeling Kit, No Dye, Agilent, Cat# 5190-0447. Agilent Gene Expression Hybridization Kit, Agilent, Cat# 5188-5242. Magic Red Cathepsin B Kit, Immunochemistry Technologies, Cat# 937. Deposited Data …”

 

Lima TS, Gov L, Lodoen MB. Evasion of Human Neutrophil-Mediated Host Defense during Toxoplasma gondii Infection. MBio. 2018 Feb 13;9(1). pii: e02027-17. doi: 10.1128/mBio.02027-17. Full text

“To directly evaluate caspase-1 enzymatic activity under each condition, we used the FAM-FLICA (ImmunoChemistry Technologies) probe on neutrophils treated with LPS and/or infected with tdTomato-expressing T. gondii and analyzed the cells by ImageStream flow cytometry.”

 

Karasawa T, Kawashima A, Usui-Kawanishi F, Watanabe S, Kimura H, Kamata R, Shirasuna K, Koyama Y, Sato-Tomita A, Matsuzaka T, Tomoda H, Park SY, Shibayama N, Shimano H, Kasahara T, Takahashi M. Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages. Arterioscler. Thromb. Vasc. Biol. 2018 Feb 8. pii: ATVBAHA.117.310581. doi: 10.1161/ATVBAHA.117.310581. [Epub ahead of print]. Abstract

“Caspase-1 activity was analyzed using the carboxyfluorescein FLICA Caspase-1 Assay kit (98; Immunochemistry Technologies, Bloomington, MN) according to the manufacturer’s instructions. Nuclei were costained with Hoechst33342 …”

 

Yuan X, Wang L, Bhat OM, Lohner H, Li PL. Differential effects of short chain fatty acids on endothelial Nlrp3 inflammasome activation and neointima formation: Antioxidant action of butyrate. Redox Biol. 2018 Feb 13;16:21-31. doi: 10.1016/j.redox.2018.02.007. [Epub ahead of print]. Full text

“This assay used Fluorescent Labeled Inhibitor of Caspases (FLICATM) probes (ImmunoChemistry Technologies, LLC, Bloomington, MN, USA) to label activated caspase-1 enzyme in the coronary arterial endothelium.”

 

Lötzerich M, Roulin PS, Boucke K, Witte R, Georgiev O, Greber UF. Rhinovirus 3C protease suppresses apoptosis and triggers caspase-independent cell death. Cell Death Dis. 2018 Feb 15;9(3):272. doi: 10.1038/s41419-018-0306-6. Full text 

“… xin V-FITC (Miltenyi Biotec) and annexin V-APC (Biolegend) kits according to the manufacturer’s instructions. Caspase-8 and 9 activities were analyzed using carboxyfluorescein FLICA caspase assays (Immunochemistry Technologies, LCC) according to the instructions of the manufacturer. Puromycin (5 μg/ml) and poly I:C (5 μg/ml) served as positive controls for apoptosis. Fluorescence was …”

 

Jelinek A, Heyder L, Daude M, Plessner M, Krippner S, Grosse R, Diederich WE, Culmsee C. Mitochondrial rescue prevents glutathione peroxidase-dependent ferroptosis. Free Radic. Biol. Med. 2018 Mar;117:45-57. doi: 10.1016/j.freeradbiomed.2018.01.019. Epub 2018 Jan 31. Abstract

“For analyzing changes in the MMP in HT22 WT or MEF cells after RSL3 challenge, the MitoPT TMRE Kit (Immunochemistry Technologies, Germany) was used.”

 

Aran G, Sanjurjo L, Bárcena C, Simon-Coma M, Téllez É, Vázquez-Vitali M, Garrido M, Guerra L, Díaz E, Ojanguren I, Elortza F, Planas R, Sala M, Armengol C, Sarrias MR. CD5L is upregulated in hepatocellular carcinoma and promotes liver cancer cell proliferation and antiapoptotic responses by binding to HSPA5 (GRP78). FASEB J. 2018 Feb 20:fj201700941RR. doi: 10.1096/fj.201700941RR. [Epub ahead of print].  Abstract

” … (MilliporeSigma) for 24 h and then detached with Accutase (Mil- liporeSigma); caspase 3/7 activity was then analyzed with an FAM-FLICA caspase 3/7 detection kit (ImmunoChemistry Technologies, Bloomington, MN, USA) according to the manufacturer’s …”

 

Products Used:

Hoechst 33342 Fluorescent Nucleic Acid Stain

Magic Red Cathepsin B Assay

FAM-FLICA Caspase-1 Assay

FAM-FLICA Caspase-8 Assay

FAM-FLICA Caspase-9 Assay

MitoPT TMRE Assay

FAM-FLICA Caspase-3/7 Assay