In January, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of January citations from 2018.

Czimmerer Z, Daniel B, Horvath A, Rückerl D, Nagy G, Kiss M, Peloquin M, Budai MM, Cuaranta-Monroy I, Simandi Z, Steiner L, Nagy B, Poliska S, Banko C, Bacso Z, Schulman IG, Sauer S, Deleuze JF, Allen JE, Benko S, Nagy L. The Transcription Factor STAT6 Mediates Direct Repression of Inflammatory Enhancers and Limits Activation of Alternatively Polarized Macrophages. Immunity. 2018. Jan 16;48(1):75-90.e6. doi: 10.1016/j.immuni.2017.12.010. Full text

“Sub-vital staining was performed in culture medium at room temperature for 20 minutes by Hoechst 34580 (10 microg/ml), propidium iodine (10 microg/ml), Alexa 488 tagged Annexin V (1 microg/ml) and caspase-1 specific FLICA® 660 (FLICA® 660 far-red fluorescence Caspase-1 Assay Kit was used according to the description of manufacturer; ImmunoChemistry Technologies, LLC).”

 

Festa BP, Chen Z, Berquez M, Debaix H, Tokonami N, Prange JA, Hoek GV, Alessio C, Raimondi A, Nevo N, Giles RH, Devuyst O, Luciani A. Impaired autophagy bridges lysosomal storage disease and epithelial dysfunction in the kidney. Nat Commun. 2018. Jan 11;9(1):161. doi: 10.1038/s41467-017-02536-7. Full text

“The detection of lysosomal activity was performed in live mPTCs using Bodipy-FL-PepA (P12271, Thermo Fischer Scientific) or Magic Red-(RR)2 substrate (MR-CtsB; 938, Immunochemistry Technologies) according to the manufacturer’s specifications. The cells were pulsed with 1 μM Bodipy-FL-Pepstatin A or with 1 μM Magic Red-(RR)2 in Live Cell Imaging (A14291DJ,…”

 

Laencina L, Dubois V, Le Moigne V, Viljoen A, Majlessi L, Pritchard J, Bernut A, Piel L, Roux AL, Gaillard JL, Lombard B, Loew D, Rubin EJ, Brosch R, Kremer L, Herrmann JL, Girard-Misguich F. Identification of genes required for Mycobacterium abscessus growth in vivo with a prominent role of the ESX-4 locus. Proc Natl Acad Sci U S A. 2018. Jan 30;115(5):E1002-E1011. doi: 10.1073/pnas.1713195115. Epub 2018 Jan 17. Abstract

“The FAM FLICA Caspase-1 Assay Kit (ImmunoChemistry Technologies) was used to evaluate the presence of catalytically active forms of caspase 1 p10 and p12.”

 

Kidana K, Tatebe T, Ito K, Hara N, Kakita A, Saito T, Takatori S, Ouchi Y, Ikeuchi T, Makino M, Saido TC, Akishita M, Iwatsubo T, Hori Y, Tomita T. Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer’s disease model mice. EMBO Mol Med. 2018. Jan 8. pii: e8184. doi: 10.15252/emmm.201708184. [Epub ahead of print]. Full text

“… ion was 20 μM), lipopolysaccharide from _Escherichia coli_ (Imgenex, stock solution was dissolved in distilled water, final concentration was 1 μg/ml), and DRAQ5™ far‐red fluorescent DNA dye (ImmunoChemistry Technologies, final concentration was 1:1,000 dilution). Memantine (stock solution was dissolved in distilled water, final concentration was 30 μM) was provided by Daiichi‐Sankyo C…”

 

Nougarede A, Popgeorgiev N, Kassem L, Omarjee S, Borel S, Mikaelian I, Lopez J, Gadet R, Marcillat O, Treilleux I, Villoutreix BO, Rimokh R, Gillet G. Breast cancer targeting through inhibition of the endoplasmic reticulum-based apoptosis regulator Nrh/BCL2L10. Cancer Res. 2018. Jan 12. pii: canres.0846.2017. doi: 10.1158/0008-5472.CAN-17-0846. [Epub ahead of print]. Abstract

“Apoptosis was assessed at the end of the corresponding drug treatment using the SR-FLICA® Poly Caspase Assay staining (ImmunoChemistry Technologies). Images were acquired using a Nikon NiE microscope and green transfected cells positive for FLICA staining were counted with a custom- made …”

 

Hover S, Foster B, Fontana J, Kohl A, Goldstein SAN, Barr JN, Mankouri J. Bunyavirus requirement for endosomal K+ reveals new roles of cellular ion channels during infection. PLoS Pathog 2018. Jan 19;14(1):e1006845. doi: 10.1371/journal.ppat.1006845. eCollection 2018 Jan. Full text 

“Cells were treated with 10 μM AG4 and 2 μg/ml Texas Red labelled EGF, Magic Red cathepsin B dye (ImmunoChemistry Technologies; ex.max 590 nm, em.max 620 nm) or 10 μg/ml pHRodo Red Dextran (Life Technologies; ex.max 560 nm, em.max 585 nm) for the indicated timepoints at 37°C.”

 

Products Used:

Hoechst 33342 Fluorescent Nucleic Acid Stain

FLICA 660 Caspase-1 Assay

Magic Red Cathepsin B Assay

FAM-FLICA Caspase-1 Assay

DRAQ5 Far-Red Fluorescent DNA Dye

SR-FLICA Poly Caspase Assay