In December, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of December citations from 2017.

Mendonça R, Ferro KP, Leonardo FC, Silva JA, Pericole FV, Saad ST, Costa FF, Conran N. Canonical Inflammasome Formation in Monocytes of Sickle Cell Anemia Patients. Blood. 

“Caspase-1 activity was determined in phenotypically-characterized monocyte populations by flow cytometry (Fam-FlicaTM kit, Immunochemistry Technologies).”

 

Clerc P, Jeanjean P, Hallalli N, Gougeon M, Pipy B, Carrey J, Fourmy D, Gigoux V. Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death. J Control Release. 2017.  Dec 1;270:120-134. doi: 10.1016/j.jconrel.2017.11.050. [Epub ahead of print].  Abstract

” … for 2 h. Immediately, 3 or 17 h after AMF exposure, cells were incubated with Fluorochrome-Labeled Inhibitors of Caspase-1 (FLICA, FAM-YVAD-FMK, excitation: 488 nm) or Caspase-3 (FLICA, FAM-DEVD-FMK, excitation: 488 nm) reagent (Immunochemistry …”

 

Mantegazza AR, Wynosky-Dolfi MA, Casson CN, Lefkovith AJ, Shin S, Brodsky IE, Marks MS. Increased autophagic sequestration in adaptor protein-3 deficient dendritic cells limits inflammasome activity and impairs antibacterial immunity. PLoS Pathog. 2017. Dec 18;13(12):e1006785. doi: 10.1371/journal.ppat.1006785. eCollection 2017 Dec. Full text 

“The caspase-1 probe FAM-YVAD-FMK (FAM-FLICA) was from ImmunoChemistry Technologies (Bloomington, MN). Recombinant LLO was purified from E. coli strain DP-3570 expressing six histidine (His6)-tagged LLO (kindly provided by Daniel Portnoy, University of California, Berkeley), and was coated onto 3-micron polystyrene beads (Polysciences Inc.) as previously described [81, 82].”

 

de Castro RJA, Siqueira IM, Jerônimo MS, Basso AMM, Veloso Junior PHH, Magalhães KG, Leonhardt LC, de Oliveira SAM, Bürgel PH, Tavares AH, Bocca AL. The Major Chromoblastomycosis Etiologic Agent Fonsecaea pedrosoi Activates the NLRP3 Inflammasome. Front Immunol. 2017. Nov 20;8:1572. doi: 10.3389/fimmu.2017.01572. eCollection 2017. Full text 

” … After 24 h of infection with F. pedrosoi conidia or hyphae, BMDMs were detached (as mentioned above) and incubated for 1 h with a caspase-1 fluorochrome-labeled inhibitor of caspases (FLICA), FAM-YVAD-FMK (Immunochemistry Technologies), according to the …”

 

Kratschmer C, Levy M. Targeted Delivery of Auristatin Modified Toxins to Pancreatic Cancer Using Aptamers. Molecular Therapy-Nucleic Acids. 2017. 10:227-236 March 2018. doi.org/10.1016/j.omtn.2017.11.013.  Full text

“Aptamers were added to cells at a final concentration of 500 μM, and Magic Red cathepsin B assay (ImmunoChemistry Technologies, Bloomington, MN) was added to cells at the manufacturer’s recommended concentration.”

 

Manshian BB, Poelmans J, Saini S, Pokhrel S, Grez JJ, Himmelreich U, Mädler L, Soenen SJ. Nanoparticle-induced inflammation can increase tumor malignancy. Acta Biomater. 2017. Dec 20. pii: S1742-7061(17)30786-9. doi: 10.1016/j.actbio.2017.12.020. [Epub ahead of print]. Abstract  

” … elsewhere [26]; [27] ; [28]. 2.6. Caspase-1 activation. Active caspase-1 was detected fluorometrically using the caspase-1 flurochrome inhibitor of caspases (FLICA) kit from Immunochemistry Technologies. Briefly, LPS-primed …”

 

Dautova Y, Kapustin AN, Pappert K, Epple M, Okkenhaug H, Cook SJ, Shanahan CM, Bootman MD, Proudfoot D. Calcium phosphate particles stimulate interleukin-1β release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release. J. Mol. Cell. Cardiol. 2017. Dec 20;115:82-93. doi: 10.1016/j.yjmcc.2017.12.007. [Epub ahead of print]. Full text 

“… or without CaP particles for up to 16 h. Culture medium was then replaced with a fluorescent caspase-1 inhibitor peptide, FAM-YVAD-FLICA, for 1 h at 37°C following the manufacturer’s recommended protocol (FAM-FLICA caspase-1 assay kit, ImmunoChemistry …”

 

Schell SL, Soni C, Fasnacht MJ, Domeier PP, Cooper TK, Rahman ZSM. Mer Receptor Tyrosine Kinase Signaling Prevents Self-Ligand Sensing and Aberrant Selection in Germinal Centers. J. Immunol. Dec 15;199(12):4001-4015. doi: 10.4049/jimmunol.1700611. Epub 2017 Nov 8. Abstract

” To detect AC accumulation, the SR-FLICA Poly Caspase Assay kit was purchased from ImmunoChemistry Technologies and the protocol was performed according to the manufacturer’s instructions.”

 

Products Used:

FAM-FLICA Caspase-1 Assay

FAM-FLICA Caspase-3/7 Assay

Magic Red Cathepsin B Assay

SR-FLICA Poly Caspase Assay