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In October, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of October citations from 2017.

Dang EV, McDonald JG, Russell DW, Cyster JG. Oxysterol Restraint of Cholesterol Synthesis Prevents AIM2 Inflammasome Activation. Cell. 2017. Nov 16.171(5):1057-1071.e11. doi: 10.1016/j.cell.2017.09.029. Epub 2017 Oct 12. Summary

“BMDMs (1×105 ) were seeded on round glass coverslips in 24-well plates overnight and then stimulated with LPS for 8 hr along with 150X FLICA reagent (ImmunoChemistry Technologies).”

 

Swanson KV, Junkins RD, Kurkjian CJ, Holley-Guthrie E, Pendse AA, El Morabiti R, Petrucelli A, Barber GN, Benedict CA, Ting JP. A noncanonical function of cGAMP in inflammasome priming and activation. J. Exp. Med. 2017. Dec 4;214(12):3611-3626. doi: 10.1084/jem.20171749.  Epub 2017 Oct 13. Abstract

“When used, active caspase-1 was visualized by the addition of FLICA-660 caspase-1 assay kit (9122; ImmunoChemistry Technologies) for the final hour during the transfection, following the manufacturer’s protocol.”

 

Qian Q, Zhang Z, Orwig, A, Chen S, Ding WX, Xu Y, Kunz RC, Lind NRL, Stamler JS, Yang L. S-nitrosoglutathione Reductase Dysfunction Contributes to Obesity-Associated Hepatic Insulin Resistance via Regulating Autophagy. Diabetes. 2017. Oct 26. pii: db170223. doi: 10.2337/db17-0223. [Epub ahead of print]. Abstract

” …The HexB activity assay was performed as described by Vaidyanathan et al. (28). The CTSB assay was performed using a CTSB Detection Kit (ImmunoChemistry Technologies), and lysosomal pH was measured using LysoSensr Green DND-189 (Thermo Fisher Scientific …”

 

Rehklau K, Hoffmann L, Gurniak CB, Ott M, Witke W, Scorrano L, Culmsee C, Rust MB. Cofilin1-dependent actin dynamics control DRP1-mediated mitochondrial fission. Cell Death Dis. 2017. Oct 5;8(10):e3063. doi: 10.1038/cddis.2017.448. Abstract

” … overnight at 4 °C. Mitochondrial membrane potential. To quantify membrane potential, cells. were stained with the potentiometric fluorescent tetramethylrhodamine ethyl ester. (TMRE) dye (MitoPT TMRE kit; Immunochemistry Technologies, Bloomington, MN,. USA).”

 

Sonntag KC, Ryu WI, Amirault KM, Healy RA, Siegel AJ, McPhie DL, Forester B, Cohen BM. Late-onset Alzheimer’s disease is associated with inherent changes in bioenergetics profiles. Sci Rep. 2017. Oct 25;7(1):14038. doi: 10.1038/s41598-017-14420-x. Abstract

“…NADH). MitoPT JC-1 Assay. The mitoPT JC-1 assay (ImmunoChemistry Technologies, Bloomington, MN) using. the slow, lipophilic, cationic fluorescent dye 5,5′,6,6′-tetrachloro-1,1′,3,3′ tetraethylbenzimidazolocarbocyanine…”

 

Products Used:

FAM-FLICA Caspase-1 Assay

FLICA 660 Caspase-1 Assay

Magic Red Cathepsin B Assay

MitoPT TMRE Assay

MitoPT JC-1 Assay