In July, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of July citations from 2017.

Ríos-Luci C, García-Alonso S, Díaz-Rodríguez E, Nadal-Serrano M, Arribas J, Ocaña A, Pandiella A. Resistance to the antibody-drug conjugate T-DM1 is based in a reduction in lysosomal proteolytic activity. Cancer Res. 2017 Jul 7. doi: 10.1158/0008-5472.CAN-16-3127. [Epub ahead of print]. Abstract.

“Cells were collected and acquired using an Accuri C6 Flow Cytometer to quantify FL2H-positive cells. Cathepsin B enzymatic activity in live cells was measured using Magic Red Cathepsin B detection kit (Immunochemistry Technologies, LLC, Bloomington, USA), following the manufacturer’s instructions.”

 

Zou HH, Yang PP, Huang TL, Zheng XX, Xu GS. PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes. Sci Rep. 2017 Jun 27;7(1):4261. doi: 10.1038/s41598-017-00686-8. Full Text.

” – … Biotechnology, Shanghai, China) for 20 min in dark at 37 °C. For mitochondria membrane potential (MMP) assay, mouse podocytes were incubated with 0.5 ml Tetrechloro-tetraethylbenzimidazol carbocyanine iodide (JC-1, Immunochemistry Technologies, …”

 

 

Shen L, Yang Y, Ou T, Key CC, Tong SH, Sequeira RC, Nelson JM, Nie Y, Wang Z, Boudyguina E, Shewale SV, Zhu X. Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy. J Lipid Res. 2017 Jul 20. pii: jlr.M075879. doi: 10.1194/jlr.M075879. [Epub ahead of print].  Full Text.

“Blood cells were then incubated with FLICA 660-YVAD-FMK (ImmunoChemistry Technologies, #9122) at 1:150 dilution in RPMI-1640 medium plus 5% FBS at 37oC for 1 h, followed by staining for monocytes and neutrophils using a cocktail of antibodies against CD45-APC (BD Pharmingen # 559864)”

 

 

Swan ZD, Bouwer AL, Wonderlich ER, Barratt-Boyes SM. Persistent accumulation of gut macrophages with impaired phagocytic function correlates with SIV disease progression in macaques. Eur. J. Immunol. 2017 Jul 1. doi: 10.1002/eji.201646904. [Epub ahead of print]. Abstract.

” – … Detection of active caspase-1 was carried out using the FAM-FLICA Caspase-1 Assay Kit (ImmunoChemistry Technologies) per the manufacturer’s instructions. To measure responses to stimulation, freshly isolated intestinal and mesenteric LN single cell…”

 

 

Boraschi-Diaz I, Tauer JT, El Rifai O, Guillemette D, Lefebvre G, Rauch F, Ferron M, Komarova SV. Metabolic phenotype in the mouse model of osteogenesis imperfecta. J. Endocrinol. 2017 Sep;234(3):279-289. doi: 10.1530/JOE-17-0335. Epub 2017 Jul 17. Abstract.

” – … 2010b). Briefly, Antibody Coating Buffer (CB1), ELISA Wash Buffer (WB1), 120 General Blocker Buffer (BB1), General Assay Diluent (AD1) and Stop Solution for TMB 121 (STOP1) were all obtained from ImmunoChemistry Technologies. 1-Step™ Ultra TMB ELISA 122 … ”

 

Products Used:

Magic Red Cathepsin B Assay Kit (Catalog #937, 938)

MitoPT JC-1 Assay (Catalog #924, 911)

FLICA 660 Caspase-1 Assay Kit (Catalog #9122)

FAM-FLICA Caspase-1 Assay Kit (Catalog #97, 98)

Antibody Coating Buffer, 5X (Catalog #644, 645, 646, 658)

ELISA Wash Buffer, 10X (Catalog# 650, 651, 652, 676)

General Block ELISA Blocking Buffer (Catalog #632, 633, 640, 659)

General Assay Diluent (Catalog #620, 621, 622, 671)

Stop Solution for TMB Substrates (Catalog #6282, 6343)