In June, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of June citations from 2017.

Ferris ST, Zakharov PN, Wan X, Calderon B, Artyomov MN, Unanue ER, and Carrero JA. The islet-resident macrophage is in an inflammatory state and senses microbial products in blood. J. Exp. Med. 2017. Jun 19. pii: jem.20170074. doi: 10.1084/jem.20170074. [Epub ahead of print]. Abstract.

“Islets were isolated and dispersed as described previously. Cells were incubated for 30 min at 37°C with the fluorescent inhibitor probe FAM-YVAD-FMK (Immunochemistry Technologies) to label active caspase-1 enzyme.”

 

Heit A, Schmitz F, Gerdts S, Flach B, Moore MS, Perkins JA, Robins HS, Aderem A, Spearman P, Tomaras GD, De Rosa SC, and McElrath MJ. Vaccination establishes clonal relatives of germinal center T cells in the blood of humans. J. Exp. Med. 2017. Jul 3;214(7):2139-2152. doi: 10.1084/jem.20161794. Epub 2017 Jun 21. Abstract.

“ELISA plates were coated with 2 µg/ml of F(ab)’2 goat anti– human IgG (Fcγ-specific) antibody (Thermo Fisher Scientific) in coating buffer (Immunochemistry Technologies).”

 

Palacios-Macapagal D, Connor J, Mustelin T, Ramalingam TR, Wynn TA, and Davidson TS. Cutting Edge: Eosinophils Undergo Caspase-1-Mediated Pyroptosis in Response to Necrotic Liver Cells. J. Immunol. 2017. Aug 1;199(3):847-853. doi: 10.4049/jimmunol.1601162. Epub 2017 Jun 26. Abstract.

“… Cells were stained with Siglec F PE (BD Pharmingen), CCR3 APC (BD Pharmingen), CD107a Pacific Blue (eBioscience), aqua live/dead Amcyan (Life Technologies), FAM-FLICA Caspase-1 (ImmunoChemistry Technologies), and FLICA 660 Caspase 3/7 far-red fluorescence …”

 

Okamoto A, Sumi C, Tanaka H, Kusunoki M, Iwai T, Nishi K, Matsuo Y, Harada H, Takenaga K, Bono H, and Hirota K. HIF-1-mediated suppression of mitochondria electron transport chain function confers resistance to lidocaine-induced cell death. Sci Rep. 2017. Jun 19;7(1):3816. doi: 10.1038/s41598-017-03980-7. Full Text.

“Mitochondrial membrane potential was assessed by flow cytometry with MitoPT JC-1 Assay Kit (ImmunoChemistry Technologies, Bloomington, MN, USA), according to the manufacturer’s instructions. Cells were seeded in 6-well plates (3 × 10^5 cells/well) and cultured overnight.”

 

Dongre A, Clements D, Fisher AJ, and Johnson SR. Cathepsin K in Lymphangioleiomyomatosis: LAM Cell-Fibroblast Interactions Enhance Protease Activity by Extracellular Acidification. Am. J. Pathol. 2017. Aug;187(8):1750-1762. doi: 10.1016/j.ajpath.2017.04.014. Epub 2017 Jun 15. Abstract.

“… peroxidase conjugate (Sigma). Cathepsin K Activity Assays. Intracellular cathepsin K activity was recorded in live cells using the Magic Red substrate (ImmunoChemistry Technologies, 2B Scientific, Bicester, UK). Cultured cells …”

 

Gornowicz A, Pawłowska N, Czajkowska A, Czarnomysy R, Bielawska A, Bielawski K, Michalak O, Staszewska-Krajewska O, and Kałuża Z. Biological evaluation of octahydropyrazin[2,1-a:5,4-a’]diisoquinoline derivatives as potent anticancer agents. Tumour Biol. 2017. Jun;39(6):1010428317701641. doi: 10.1177/1010428317701641. Full Text.

“Caspase-8 activity was measured using the FAM-FLICA Caspase 8 Kit (ImmunoChemistry Technologies, Bloomington, MN, USA) according to the manufacturer’s instructions.”

 

Products Used:

FAM-FLICA Caspase-1 Assay Kit (Catalog #97, 98)

Antibody Coating Buffer, 5X (Catalog #644, 645, 646, 658)

MitoPT JC-1 Assay (Catalog #924, 911)

Magic Red Cathepsin K Assay Kit (Catalog #939, 940)

FAM-FLICA Caspase-8 Assay Kit (Catalog #99, 910)