In May, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of May citations from 2017.

 

Calvier L, Chouvarine P, Legchenko E, Hoffmann N, Geldner J, Borchert P, Jonigk D, Mozes MM, and Hansmann G. PPARγ Links BMP2 and TGFβ1 Pathways in Vascular Smooth Muscle Cells, Regulating Cell Proliferation and Glucose Metabolism. Cell Metab. 2017. May 2;25(5):1118-1134.e7. doi: 10.1016/j.cmet.2017.03.011. Abstract.

“MitoPT JC-1 Assay kit, Immunochemistry Technologies, Cat#911.”

 

Sakamaki JI, Wilkinson S, Hahn M, Tasdemir N, O’Prey J, Clark W, Hedley A, Nixon C, Long JS, New M, Van Acker T, Tooze SA, Lowe SW, Dikic I, and Ryan KM. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol. Cell. 2017. May 18;66(4):517-532.e9. doi: 10.1016/j.molcel.2017.04.027. Full text.

“To measure lysosomal Cathepsin B activity, cells were incubated with Magic Red CathepsinB (ImmunoChemistry Technologies, Cat#: 938) for 1 hr according to the manufacturer’s instructions.”

 

Bednash JS, Weathington N, Londino J, Rojas M, Gulick DL, Fort R, Han S, McKelvey AC, Chen BB, and Mallampalli RK. Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity. Nat Commun. 2017. May 11;8:15203. doi: 10.1038/ncomms15203. Full text.

“THP-1 cells (1 x 10^6 cells per mL) were pre-treated with BC-1471 (10 mg/mL) for 16 h prior to addition of LPS (500 ng/ml), Pam3CSK4 (100 ng/ml) or control for 6 h. FAM-FLICA reagent (Immunochemistry Technologies, 98) was added 4 h before the end of the assay, prepared according to the manufacturer’s protocol.”

 

Guo C, Zhu Z, Guo Y, Wang X, Yu P, Xiao S, Chen Y, Cao Y, and Liu X. Heparanase upregulation contributes to porcine reproductive and respiratory syndrome virus release. J. Virol. 2017. May 10. pii: JVI.00625-17. doi: 10.1128/JVI.00625-17. [Epub ahead of print]. Abstract.

” Enzymatic activity assay Cathepsin L activity in living cells was monitored using the Magic Red Cathepsin L detection kit (ImmunoChemistry Technologies, MN, USA). Briefly, cells were seeded onto coverslips, and then inoculated with PRRSV at an multiplicity of infection (MOI) of 1, 2, or 3. After incubation for the indicated times, Magic Red staining solution which is a non-cytotoxic substrate that fluoresces red upon cleavage by active cathepsins was added for 1 h at 37 °C. ”

 

Products Used:

MitoPT JC-1 Assay Kit (Catalog #924, 911)

Magic Red Cathepsin B Assay Kit (Catalog #938, 939)

FAM-FLICA Caspase-1 Assay Kit (Catalog #97, 98)

Magic Red Cathepsin L Assay Kit (Catalog #941, 942)