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In April, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of April citations from 2017.

Kang HT, Park JT, Choi K, Kim Y, Choi HJC, Jung CW, Lee YS, Chul Park S. Chemical screening identifies ATM as a target for alleviating senescence. Nat Chem Biol. 2017. Jun;13(6):616-623. Epub 2017 Mar 27. Abstract.

“Cathepsin activity was determined with MagicRed cathepsin B (CTSB) and cathepsin L (CTSL) assay kits (MR-CTSB and MR-CTSL, respectively; ImmunoChemistry Technologies), according to the manufacturer’s instructions.”

 

Ishii T, Hosoki K, Nikura Y, Yamashita N, Nagase T, Yamashita N. IFN Regulatory Factor 3 Potentiates Emphysematous Aggravation by Lipopolysaccharide. J. Immunol. 2017. May 1;198(9):3637-3649. Epub 2017 Mar 31. Abstract. 

“Lung neutrophils (2.5 3 105 cells per tube) that had not been affected by PI were incubated with FITC-labeled annexin V and PI in an apoptosis detection kit (Takara, Tokyo, Japan) or with MitoPT in a MitoPT apoptosis detection kit (ImmunoChemistry Technologies, Bloomington, MN) and analyzed by flow cytometry (SH800 cell sorter; Sony).”

 

Boudoures AL, Saben J, Drury A, Scheaffer S, Modi Z, Zhang W, Moley, KH. Obesity-exposed oocytes accumulate and transmit damaged mitochondria due to an inability to activate mitophagy. Dev Biol. 2017. Jun 1;426(1):126-138. Epub 2017 Apr 21. Full text.

“For JC-1 staining of oocytes, the MitoPT™ JC-1 Assay Kit (Immunochemistry Technologies, Bloomington, MN) was used according to the manufacturer’s protocol with the following modification: oocytes were stained for 20 min in 200 µl of JC-1 diluted in M2 medium instead of assay buffer followed by a single manufacturer prescribed wash once for 5 min in pre-warmed EmbryoMax FHM with HEPES without phenol red (MR-025-D; EMD Millipore) with 5 µM milrinone and 10% FBS.”

 

Niu H, Klem T, Yang J, Qiu Y, Pan L. A biotin-drug extraction and acid dissociation (BEAD) procedure to eliminate matrix and drug interference in a protein complex anti-drug antibody (ADA) isotype specific assay. J Immunol Methods. 2017. Jul;446:30-36. Epub 2017 Apr 5. Abstract.

” –  … Sulfo-tag™-conjugated anti-human/NHP IgG or IgM Ab was purchased from MSD (Meso Scale Discovery, Rockville, Maryland). IgM-reducing assay diluent was purchased from ImmunoChemistry Technologies (Bloomington, MN). … ”

 

Products Used:

Magic Red Cathepsin B Assay Kit (Catalog #938, 939)

Magic Red Cathepsin L Assay Kit (Catalog #941, 942)

MitoPT JC-1 Assay Kit (Catalog #924, 911)

IgM-Reducing Assay Diluent (Catalog #623, 624, 625, 672)