In August, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of publications from August 2016.

 

Chailangkarn T, Trujillo CA, Freitas BC, Hrvoj-Mihic B, Herai RH, Yu DX, Brown TT, Marchetto MC, Bardy C, McHenry L, Stefanacci L, Järvinen A, Searcy YM, DeWitt M, Wong W, Lai P, Ard MC, Hanson KL, Romero S, Jacobs B, Dale AM, Dai L, Korenberg JR, Gage FH, Bellugi U, Halgren E, Semendeferi K,Muotri AR. A human neurodevelopmental model for Williams syndrome. Nature. 536(7616): 338-343. Abstract.

“Caspase activity was measured using a Green FLICA Caspases 3 & 7 Assay Kit (ImmunoChemistry Technologies).”

 

Bright NA, Davis LJ, Luzio JP. Endolysosomes Are the Principal Intracellular Sites of Acid Hydrolase Activity. Curr. Biol. 26(17):  2233-2245. Full text.

“To label endocytic organelles in which cathepsin B or cathepsin L was catalytically active, cells were incubated with the respective Magic Red substrates from ImmunoChemistry Technologies, at a final concentration of 673 nM.”

 

Liu T, Tang Q, Liu K, Xie W, Liu X, Wang H, Wang RF, Cui J. TRIM11 Suppresses AIM2 Inflammasome by Degrading AIM2 via p62-Dependent Selective Autophagy. Cell Rep. 16(7): 1988-2002. Full text.

“The levels of active caspase-1 were quantified using a fluorescent probe (fluorochrome -labeled inhibitors of caspases [FLICA] FAM-YVAD-FMK, ImmunoChemistry Technologies) that specifically recognized active caspase-1.”

 

Kameyama H, Nakajima H, Nishitsuji K, Mikawa S, Uchimura K, Kobayashi N, Okuhira K, Saito H, Sakashita N. Iowa Mutant Apolipoprotein A-I (ApoA-IIowa) Fibrils Target Lysosomes. Sci Rep. 6: 30391. Full text.

“Mitochondrial membrane potential was determined by using the mitochondrial membrane potential-sensitive fluorophore tetramethylrhodamine ethyl ester (TMRE) (MitoPT assay kit; ImmunoChemistry Technologies, Bloomington, MN).”

 

Rogozhnikov D, Luo W, Elahipanah S, O’Brien PJ, Yousaf MN. Generation of a Scaffold-Free Three-Dimensional Liver Tissue via a Rapid Cell-to-Cell Click Assembly Process. Bioconjug Chem. 2016. Abstract.

“To visualize the secretion of ECM by tissues over time, the cells were stained with the green fluorescent probe Col-F (Immunochemistry Technologies, MN), which binds collagen and elastin. A 20 mM stock solution of Col-F reagent in DMSO was prepared.”

 

Products Used:

FAM-FLICA Caspase 3 & 7 Assay Kit (Catalog #93, 94)

Magic Red Cathepsin B Assay Kit (Catalog #937, 938)

Magic Red Cathepsin L Assay Kit (Catalog #941, 942)

FAM-FLICA Caspase 1 Assay Kit (Catalog #97, 98)

MitoPT TMRE Assay Kit (Catalog #9103)

Col-F Collagen Binding Reagent (Catalog #6346)