In July, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of publications from July 2016. Nabekura T, Lanier LL. Activating Receptors for Self-MHC Class I Enhance Effector Functions and Memory Differentiation of NK Cells during Mouse Cytomegalovirus Infection. Immunity. 45(1): 74-82. Abstract. "For measuring apoptosis, we stained cells by using the FAM FLICA poly caspase assay kit (ImmunoChemistry Technologies) according to the manufacturer’s protocol and with antibodies against cell surface molecules. Samples were acquired on an LSRII or a FACSCalibur (BD Biosciences) and analyzed with FlowJo software (Tree Star)." Trudeau KM, Colby AH, Zeng J, Las G, Feng JH, Grinstaff MW, Shirihai OS. Lysosome acidification by photoactivated nanoparticles restores autophagy under lipotoxicity. J. Cell. Biol. 214(1): 25-34. Abstract. "Cells were stained with 10 µg/ml Magic red cathepsin L (MR-cathepsin L; Immunochemistry Technologies) for 1 h. The cells were then washed three times with PBS and imaged using Celigo Imaging Cell Cytometer (Brooks Life Science Systems)." Conroy MJ, Fitzgerald V, Doyle SL, Channon S, Useckaite Z, Gilmartin N, O'Farrelly C, Ravi N, Reynolds JV, Lysaght J. The microenvironment of visceral adipose tissue and liver alter natural killer cell viability and function. J. Leukoc. Biol. 2016. Abstract. "... mediated cytotoxicity assay Natural and IL-2-induced cytotoxicity by PBMCs from 3 healthy donors against the myeloid erythroleukemia-derived cell line K562 cells was measured using the Cell-Mediated Cytotoxicity Assay (ImmunoChemistry Technologies)." Products used: FAM-FLICA Poly Caspase Assay Kit (Catalog #91, 92) Magic Red Cathepsin L Assay Kit (Catalog #941, 942) Cell-Mediated Cytotoxicity Assay: Total Cytotoxicity Test (Catalog #971, 972)
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