In May, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of publications from May 2016.

 

Zhang C, Xin H, Zhang W, Yazaki PJ, Zhang Z, Le K, Li W, Lee H, Kwak L, Forman S, Jove R, Yu H. CD5 Binds to Interleukin-6 and Induces a Feed-Forward Loop with the Transcription Factor STAT3 in B Cells to Promote Cancer. Immunity. 44(4):  913-923. Abstract.

“To detect IL-6 binding to CD5, we performed enzyme-linked immunosorbent assay (Ieguchi et al., 2010). In brief, recombinant mouse CD5 was incubated in a 96-well plate at 4°C for overnight. Unbound CD5 was removed, and 200 μL of blocking buffer (ImmunoChemistry Technologies) was added and incubated at RT for 2 hr. The wells were washed with PBS containing 0.05% Tween-20, and soluble IL-6 in 1% BSA was added to each well and incubated at room temperature for 2 hr. IL-6 was quantified with biotin-avidin assay followed by a colorimetric detection. Anti-mCD5 blocking assay was performed as described above, except that anti-CD5 antibody was added to CD5-coated wells before adding IL-6.”

 

Shah A, Kannambath S, Herbst S, Rogers A, Soresi S, Carby M, Reed A, Mostowy S, Fisher MC, Shaunak S, Armstrong-James DP. Calcineurin Orchestrates Lateral Transfer of Aspergillus fumigatus During Macrophage Cell Death. Am. J. Respir. Crit. Care. Med. 2016. Abstract.

” Imaging of activated Cathepsin B and phagosomal acidification was performed on live cells using a Magic Red Cathepsin B staining kit (Immunochemistry Technologies)…”

 

O’Sullivan TE, Geary CD, Weizman OE, Geiger TL, Rapp M, Dorn GW 2nd, Overholtzer M, Sun JC. Atg5 Is Essential for the Development and Survival of Innate Lymphocytes. Cell Rep. 15(9):  1910-1919. Full text.

“Pan-caspase staining was carried out using the carboxyfluorescein FLICA in vitro Poly Caspase Kit (ImmunoChemistry Technologies).”

 

Kulabaş N, Tatar E, Bingöl Özakpınar Ö, Özsavcı D, Pannecouque C, De Clercq E, Küçükgüzel İ. Synthesis and antiproliferative evaluation of novel 2-(4H-1,2,4-triazole-3-ylthio)acetamide derivatives as inducers of apoptosis in cancer cells. Eur J Med Chem. 121: 58-70. Abstract.

“The loss of mitochondrial membrane potential was detected by JC-1 mitochondrial membrane potential (MMP) kit (MitoPT JC-1, ImmunoChemistry Technologies, LLC).”

 

Products used:

General Block ELISA Blocking Buffer (Catalog #632, 633, 640, 659)

Magic Red Cathepsin B Assay Kit (Catalog #937, 938)

FAM FLICA Poly Caspase Assay Kit (Catalog #91, 92)

MitoPT JC-1 Assay Kit (Catalog #924, 911)