Universal ELISA Wash Buffer (WB1)

Use WB1 to rinse microtiter plates during the coating process and between reagent addition steps of an ELISA. It is a universal ELISA wash buffer: it may be used with antibody-sandwich ELISAs and antigen-down ELISAs.

ICT’s ELISA Wash Buffer formulation is an optimal formulation of pH stabilizers, salts, and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background noise and increasing the specific signal. ICT’s 10x ELISA Wash Buffer is compatible with all routinely used conjugate components such as HRP, AP, and avidin, among others.

ELISA Wash Buffer

Available Volumes and Pricing

Store WB1 at Room Temperature. Product may ship overnight, 2-Day, or Express Saver without ice; only overnight shipping is available for orders placed online.

FAQ

How much WB1 do I need?

Each wash will use about 30-40mL per plate. If you are washing 3 times between steps of your ELISA, you will use about 120mL of 1x wash buffer per wash cycle. With 2 wash cycles, that's about 240mL per ELISA.

How do I use WB1?

Simply dilute WB1 1:10 (for example, add 100mL WB1 to 900mL diH20 for a final volume of 1000mL), let the solution stir for 15 minutes, and wash your plate. As WB1 is concentrated, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm or mix the buffer until all crystals are dissolved. Do not let it boil.

How do I wash plates using WB1?

To wash microtiter plates, the diluted wash buffer may be dispensed through a squirt bottle, a plate washer, through a multi-channel pipette, or through an automated system. If washing by hand, be sure to fill the wells all the way up with 1x wash buffer (about 400uL/well). Then aspirate or dump out the buffer and repeat for a total of 2-4 washes. After the final dump or aspiration, pound the plate on paper towels to remove any excess liquid. We do not recommend submerging the entire plates in a bath as many of the wells will become contaminated, which decreases the reliability of the assay.

If you are washing the plate during the coating process, we recommend 2-3 washes between steps. If you are washing the plate as part of an ELISA procedure, we recommend 2-4 washes between steps.